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Genomic DNA Extracted from Lactiplantibacillus plantarum Attenuates Porphyromonas gingivalis Lipopolysaccharide (LPS)-Induced Inflammatory Responses via Suppression of Toll-Like Receptor (TLR)-Mediated Mitogen-Activated Protein Kinase (MAPK) and Nuclear Factor-κB (NF-κB) Signaling Pathways
In the present study, we aimed to examine the inhibition of genomic DNA from Lactiplantibacillus plantarum (LpDNA) on Porphyromonas gingivalis lipopolysaccharide (PgLPS)-induced inflammatory responses in RAW264.7 cells. Pretreatment with LpDNA for 15 h significantly inhibited PgLPS-induced mRNA expr...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society for Food Science of Animal Resources
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493568/ https://www.ncbi.nlm.nih.gov/pubmed/37701749 http://dx.doi.org/10.5851/kosfa.2023.e43 |
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author | Choi, Young Hyeon Kim, Bong Sun Kang, Seok-Seong |
author_facet | Choi, Young Hyeon Kim, Bong Sun Kang, Seok-Seong |
author_sort | Choi, Young Hyeon |
collection | PubMed |
description | In the present study, we aimed to examine the inhibition of genomic DNA from Lactiplantibacillus plantarum (LpDNA) on Porphyromonas gingivalis lipopolysaccharide (PgLPS)-induced inflammatory responses in RAW264.7 cells. Pretreatment with LpDNA for 15 h significantly inhibited PgLPS-induced mRNA expression and protein secretion of interleukin (IL)-1β, IL-6, and monocyte chemoattractant protein-1. LpDNA pretreatment also reduced the mRNA expression of Toll-like receptor (TLR)2 and TLR4. Furthermore, LpDNA inhibited the phosphorylation of mitogen-activated protein kinases (MAPKs) and the activation of nuclear factor-κB (NF-κB) induced by PgLPS. Taken together, these findings demonstrate that LpDNA attenuates PgLPS-induced inflammatory responses by regulating MAPKs and NF-κB signaling pathways through the suppression of TLR2 and TLR4 expression. |
format | Online Article Text |
id | pubmed-10493568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Korean Society for Food Science of Animal Resources |
record_format | MEDLINE/PubMed |
spelling | pubmed-104935682023-09-12 Genomic DNA Extracted from Lactiplantibacillus plantarum Attenuates Porphyromonas gingivalis Lipopolysaccharide (LPS)-Induced Inflammatory Responses via Suppression of Toll-Like Receptor (TLR)-Mediated Mitogen-Activated Protein Kinase (MAPK) and Nuclear Factor-κB (NF-κB) Signaling Pathways Choi, Young Hyeon Kim, Bong Sun Kang, Seok-Seong Food Sci Anim Resour Short Communication In the present study, we aimed to examine the inhibition of genomic DNA from Lactiplantibacillus plantarum (LpDNA) on Porphyromonas gingivalis lipopolysaccharide (PgLPS)-induced inflammatory responses in RAW264.7 cells. Pretreatment with LpDNA for 15 h significantly inhibited PgLPS-induced mRNA expression and protein secretion of interleukin (IL)-1β, IL-6, and monocyte chemoattractant protein-1. LpDNA pretreatment also reduced the mRNA expression of Toll-like receptor (TLR)2 and TLR4. Furthermore, LpDNA inhibited the phosphorylation of mitogen-activated protein kinases (MAPKs) and the activation of nuclear factor-κB (NF-κB) induced by PgLPS. Taken together, these findings demonstrate that LpDNA attenuates PgLPS-induced inflammatory responses by regulating MAPKs and NF-κB signaling pathways through the suppression of TLR2 and TLR4 expression. Korean Society for Food Science of Animal Resources 2023-09 2023-09-01 /pmc/articles/PMC10493568/ /pubmed/37701749 http://dx.doi.org/10.5851/kosfa.2023.e43 Text en © Korean Society for Food Science of Animal Resources https://creativecommons.org/licenses/by-nc/3.0/This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Choi, Young Hyeon Kim, Bong Sun Kang, Seok-Seong Genomic DNA Extracted from Lactiplantibacillus plantarum Attenuates Porphyromonas gingivalis Lipopolysaccharide (LPS)-Induced Inflammatory Responses via Suppression of Toll-Like Receptor (TLR)-Mediated Mitogen-Activated Protein Kinase (MAPK) and Nuclear Factor-κB (NF-κB) Signaling Pathways |
title | Genomic DNA Extracted from Lactiplantibacillus
plantarum Attenuates Porphyromonas gingivalis
Lipopolysaccharide (LPS)-Induced Inflammatory Responses via Suppression of
Toll-Like Receptor (TLR)-Mediated Mitogen-Activated Protein Kinase (MAPK) and
Nuclear Factor-κB (NF-κB) Signaling Pathways |
title_full | Genomic DNA Extracted from Lactiplantibacillus
plantarum Attenuates Porphyromonas gingivalis
Lipopolysaccharide (LPS)-Induced Inflammatory Responses via Suppression of
Toll-Like Receptor (TLR)-Mediated Mitogen-Activated Protein Kinase (MAPK) and
Nuclear Factor-κB (NF-κB) Signaling Pathways |
title_fullStr | Genomic DNA Extracted from Lactiplantibacillus
plantarum Attenuates Porphyromonas gingivalis
Lipopolysaccharide (LPS)-Induced Inflammatory Responses via Suppression of
Toll-Like Receptor (TLR)-Mediated Mitogen-Activated Protein Kinase (MAPK) and
Nuclear Factor-κB (NF-κB) Signaling Pathways |
title_full_unstemmed | Genomic DNA Extracted from Lactiplantibacillus
plantarum Attenuates Porphyromonas gingivalis
Lipopolysaccharide (LPS)-Induced Inflammatory Responses via Suppression of
Toll-Like Receptor (TLR)-Mediated Mitogen-Activated Protein Kinase (MAPK) and
Nuclear Factor-κB (NF-κB) Signaling Pathways |
title_short | Genomic DNA Extracted from Lactiplantibacillus
plantarum Attenuates Porphyromonas gingivalis
Lipopolysaccharide (LPS)-Induced Inflammatory Responses via Suppression of
Toll-Like Receptor (TLR)-Mediated Mitogen-Activated Protein Kinase (MAPK) and
Nuclear Factor-κB (NF-κB) Signaling Pathways |
title_sort | genomic dna extracted from lactiplantibacillus
plantarum attenuates porphyromonas gingivalis
lipopolysaccharide (lps)-induced inflammatory responses via suppression of
toll-like receptor (tlr)-mediated mitogen-activated protein kinase (mapk) and
nuclear factor-κb (nf-κb) signaling pathways |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493568/ https://www.ncbi.nlm.nih.gov/pubmed/37701749 http://dx.doi.org/10.5851/kosfa.2023.e43 |
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