A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry

We present a protocol for measuring the pH of cell-free bacterial-conditioned media based on changes in the ultraviolet-visible (UV-Vis) absorbance spectrum using the pH indicator dye litmus. This protocol includes detailed procedures for performing bacterial culturing, examining bacterial growth, c...

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Detalles Bibliográficos
Autores principales: Engevik, Kristen A., Gonzalez, Hyland, Daniels, Clay, Stavert, Ronald K., Oezguen, Numan, Engevik, Melinda A., Horvath, Thomas D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493592/
https://www.ncbi.nlm.nih.gov/pubmed/37682718
http://dx.doi.org/10.1016/j.xpro.2023.102540
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author Engevik, Kristen A.
Gonzalez, Hyland
Daniels, Clay
Stavert, Ronald K.
Oezguen, Numan
Engevik, Melinda A.
Horvath, Thomas D.
author_facet Engevik, Kristen A.
Gonzalez, Hyland
Daniels, Clay
Stavert, Ronald K.
Oezguen, Numan
Engevik, Melinda A.
Horvath, Thomas D.
author_sort Engevik, Kristen A.
collection PubMed
description We present a protocol for measuring the pH of cell-free bacterial-conditioned media based on changes in the ultraviolet-visible (UV-Vis) absorbance spectrum using the pH indicator dye litmus. This protocol includes detailed procedures for performing bacterial culturing, examining bacterial growth, collecting cell-free supernatant, litmus dye addition, and pH-based calibration curve preparations. This assay has been designed for flexible formatting that can accommodate both high-volume and low-volume sample sets.
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spelling pubmed-104935922023-09-12 A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry Engevik, Kristen A. Gonzalez, Hyland Daniels, Clay Stavert, Ronald K. Oezguen, Numan Engevik, Melinda A. Horvath, Thomas D. STAR Protoc Protocol We present a protocol for measuring the pH of cell-free bacterial-conditioned media based on changes in the ultraviolet-visible (UV-Vis) absorbance spectrum using the pH indicator dye litmus. This protocol includes detailed procedures for performing bacterial culturing, examining bacterial growth, collecting cell-free supernatant, litmus dye addition, and pH-based calibration curve preparations. This assay has been designed for flexible formatting that can accommodate both high-volume and low-volume sample sets. Elsevier 2023-09-07 /pmc/articles/PMC10493592/ /pubmed/37682718 http://dx.doi.org/10.1016/j.xpro.2023.102540 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Engevik, Kristen A.
Gonzalez, Hyland
Daniels, Clay
Stavert, Ronald K.
Oezguen, Numan
Engevik, Melinda A.
Horvath, Thomas D.
A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry
title A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry
title_full A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry
title_fullStr A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry
title_full_unstemmed A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry
title_short A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry
title_sort high-throughput protocol for measuring solution ph of bacterial cultures using uv-vis absorption spectrophotometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493592/
https://www.ncbi.nlm.nih.gov/pubmed/37682718
http://dx.doi.org/10.1016/j.xpro.2023.102540
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