A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry

We present a protocol for measuring the pH of cell-free bacterial-conditioned media based on changes in the ultraviolet-visible (UV-Vis) absorbance spectrum using the pH indicator dye litmus. This protocol includes detailed procedures for performing bacterial culturing, examining bacterial growth, c...

Descripción completa

Detalles Bibliográficos
Autores principales: Engevik, Kristen A., Gonzalez, Hyland, Daniels, Clay, Stavert, Ronald K., Oezguen, Numan, Engevik, Melinda A., Horvath, Thomas D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493592/
https://www.ncbi.nlm.nih.gov/pubmed/37682718
http://dx.doi.org/10.1016/j.xpro.2023.102540

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493592/
https://www.ncbi.nlm.nih.gov/pubmed/37682718
http://dx.doi.org/10.1016/j.xpro.2023.102540

Ejemplares similares