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A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry

We present a protocol for measuring the pH of cell-free bacterial-conditioned media based on changes in the ultraviolet-visible (UV-Vis) absorbance spectrum using the pH indicator dye litmus. This protocol includes detailed procedures for performing bacterial culturing, examining bacterial growth, c...

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Detalles Bibliográficos
Autores principales:	Engevik, Kristen A., Gonzalez, Hyland, Daniels, Clay, Stavert, Ronald K., Oezguen, Numan, Engevik, Melinda A., Horvath, Thomas D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493592/ https://www.ncbi.nlm.nih.gov/pubmed/37682718 http://dx.doi.org/10.1016/j.xpro.2023.102540

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