Functional in vitro and in vivo analysis of biosynthetic genes by heterologous expression in E. coli

Biosynthetic gene clusters of natural products often harbor genes of unknown function, which are difficult to characterize. Here, we present a protocol for the functional analysis in vitro and in vivo of these biosynthetic genes by heterologous expression in E. coli. We describe steps for the expres...

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Detalles Bibliográficos
Autores principales: Kramer, Jil-Christine, Böhringer, Nils, Mettal, Ute, Schäberle, Till F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493593/
https://www.ncbi.nlm.nih.gov/pubmed/37682715
http://dx.doi.org/10.1016/j.xpro.2023.102531

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493593/
https://www.ncbi.nlm.nih.gov/pubmed/37682715
http://dx.doi.org/10.1016/j.xpro.2023.102531

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