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Functional in vitro and in vivo analysis of biosynthetic genes by heterologous expression in E. coli
Biosynthetic gene clusters of natural products often harbor genes of unknown function, which are difficult to characterize. Here, we present a protocol for the functional analysis in vitro and in vivo of these biosynthetic genes by heterologous expression in E. coli. We describe steps for the expres...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493593/ https://www.ncbi.nlm.nih.gov/pubmed/37682715 http://dx.doi.org/10.1016/j.xpro.2023.102531 |