Performance upgrade of a microbial explosives’ sensor strain by screening a high throughput saturation library of a transcriptional regulator

We present a methodology for a high-throughput screening (HTS) of transcription factor libraries, based on bacterial cells and GFP fluorescence. The method is demonstrated on the Escherichia coli LysR-type transcriptional regulator YhaJ, a key element in 2,4-dinitrotuluene (DNT) detection by bacteri...

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Detalles Bibliográficos
Autores principales: David, Lidor, Shpigel, Etai, Levin, Itay, Moshe, Shaked, Zimmerman, Lior, Dadon-Simanowitz, Shilat, Shemer, Benjamin, Levkovich, Shon A., Larush, Liraz, Magdassi, Shlomo, Belkin, Shimshon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493890/
https://www.ncbi.nlm.nih.gov/pubmed/37701016
http://dx.doi.org/10.1016/j.csbj.2023.08.017

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