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Role of Fibrinogen in Type-2 Diabetes Mellitus with Diabetic Neuropathy and its Preliminary Mechanism
INTRODUCTION: Diabetic peripheral neuropathy (DN) is the most common complication of type 2 diabetes mellitus (T2DM). OBJECTIVE: This study aimed to explore the role of fibrinogen (FIB) in T2DM neuropathy and its preliminary mechanism. METHODS: Ten male Sprague-Dawley rats were divided into a normal...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Bentham Science Publishers
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10494282/ https://www.ncbi.nlm.nih.gov/pubmed/37165590 http://dx.doi.org/10.2174/0929866530666230509140515 |
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author | Gu, Wei-Li Li, Zhen-Hong Zhang, Si-Qin Ao, Pian Zhu, Xiao-Bei Zhao, Xin Zhang, Xin-Yue Zhang, Deng-Feng Huang, Xiao-Juan Jiang, Yu Wei, Li |
author_facet | Gu, Wei-Li Li, Zhen-Hong Zhang, Si-Qin Ao, Pian Zhu, Xiao-Bei Zhao, Xin Zhang, Xin-Yue Zhang, Deng-Feng Huang, Xiao-Juan Jiang, Yu Wei, Li |
author_sort | Gu, Wei-Li |
collection | PubMed |
description | INTRODUCTION: Diabetic peripheral neuropathy (DN) is the most common complication of type 2 diabetes mellitus (T2DM). OBJECTIVE: This study aimed to explore the role of fibrinogen (FIB) in T2DM neuropathy and its preliminary mechanism. METHODS: Ten male Sprague-Dawley rats were divided into a normal control group (NC group) and a T2DM neuropathy model group (DN group). The DN group was given a high-energy diet and streptozotocin, while the NC group was given a normal diet and a citric acid buffer. The expression levels of related proteins were analysed. RESULTS: Electrophysiology: Compared with the NC group, the conduction latency of the somatosensory-evoked potential and nerve conduction velocity was prolonged in the DN group, while the motor nerve action potential was decreased. As seen under a light microscope, the peripheral nerve fibres in the DN group were swollen, and the nerve fibres in the posterior funiculus of the spinal cord were loose or missing. Moreover, as seen under an electron microscope, the peripheral nerve demyelination of the DN group was severe, with microvascular blood coagulation, luminal stenosis, and collapse. Compared with the NC group, in the DN group, the expression of FIB was positively correlated with the expression of both ionised calcium-binding adaptor molecule-1 and glial fibrillary acidic protein. Compared with the NC group, in the DN group, the expression of platelet/endothelial cell adhesion molecule-1 and B-cell lymphoma 2 was negatively correlated. CONCLUSION: The increased concentration of FIB may be the cause of neuropathy, and its mechanism may be related to its promotion of inflammatory response, blood coagulation, and vascular stenosis. |
format | Online Article Text |
id | pubmed-10494282 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Bentham Science Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-104942822023-09-12 Role of Fibrinogen in Type-2 Diabetes Mellitus with Diabetic Neuropathy and its Preliminary Mechanism Gu, Wei-Li Li, Zhen-Hong Zhang, Si-Qin Ao, Pian Zhu, Xiao-Bei Zhao, Xin Zhang, Xin-Yue Zhang, Deng-Feng Huang, Xiao-Juan Jiang, Yu Wei, Li Protein Pept Lett Life Sciences, Protein and Peptide Sciences, Biochemistry and Molecular Biology INTRODUCTION: Diabetic peripheral neuropathy (DN) is the most common complication of type 2 diabetes mellitus (T2DM). OBJECTIVE: This study aimed to explore the role of fibrinogen (FIB) in T2DM neuropathy and its preliminary mechanism. METHODS: Ten male Sprague-Dawley rats were divided into a normal control group (NC group) and a T2DM neuropathy model group (DN group). The DN group was given a high-energy diet and streptozotocin, while the NC group was given a normal diet and a citric acid buffer. The expression levels of related proteins were analysed. RESULTS: Electrophysiology: Compared with the NC group, the conduction latency of the somatosensory-evoked potential and nerve conduction velocity was prolonged in the DN group, while the motor nerve action potential was decreased. As seen under a light microscope, the peripheral nerve fibres in the DN group were swollen, and the nerve fibres in the posterior funiculus of the spinal cord were loose or missing. Moreover, as seen under an electron microscope, the peripheral nerve demyelination of the DN group was severe, with microvascular blood coagulation, luminal stenosis, and collapse. Compared with the NC group, in the DN group, the expression of FIB was positively correlated with the expression of both ionised calcium-binding adaptor molecule-1 and glial fibrillary acidic protein. Compared with the NC group, in the DN group, the expression of platelet/endothelial cell adhesion molecule-1 and B-cell lymphoma 2 was negatively correlated. CONCLUSION: The increased concentration of FIB may be the cause of neuropathy, and its mechanism may be related to its promotion of inflammatory response, blood coagulation, and vascular stenosis. Bentham Science Publishers 2023-07-14 2023-07-14 /pmc/articles/PMC10494282/ /pubmed/37165590 http://dx.doi.org/10.2174/0929866530666230509140515 Text en © 2023 Bentham Science Publishers https://creativecommons.org/licenses/by/4.0/© 2023 The Author(s). Published by Bentham Science Publisher. This is an open access article published under CC BY 4.0 https://creativecommons.org/licenses/by/4.0/legalcode) |
spellingShingle | Life Sciences, Protein and Peptide Sciences, Biochemistry and Molecular Biology Gu, Wei-Li Li, Zhen-Hong Zhang, Si-Qin Ao, Pian Zhu, Xiao-Bei Zhao, Xin Zhang, Xin-Yue Zhang, Deng-Feng Huang, Xiao-Juan Jiang, Yu Wei, Li Role of Fibrinogen in Type-2 Diabetes Mellitus with Diabetic Neuropathy and its Preliminary Mechanism |
title | Role of Fibrinogen in Type-2 Diabetes Mellitus with Diabetic Neuropathy and its Preliminary Mechanism |
title_full | Role of Fibrinogen in Type-2 Diabetes Mellitus with Diabetic Neuropathy and its Preliminary Mechanism |
title_fullStr | Role of Fibrinogen in Type-2 Diabetes Mellitus with Diabetic Neuropathy and its Preliminary Mechanism |
title_full_unstemmed | Role of Fibrinogen in Type-2 Diabetes Mellitus with Diabetic Neuropathy and its Preliminary Mechanism |
title_short | Role of Fibrinogen in Type-2 Diabetes Mellitus with Diabetic Neuropathy and its Preliminary Mechanism |
title_sort | role of fibrinogen in type-2 diabetes mellitus with diabetic neuropathy and its preliminary mechanism |
topic | Life Sciences, Protein and Peptide Sciences, Biochemistry and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10494282/ https://www.ncbi.nlm.nih.gov/pubmed/37165590 http://dx.doi.org/10.2174/0929866530666230509140515 |
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