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Bazi Bushen alleviates skin senescence by orchestrating skin homeostasis in SAMP6 mice

Bazi Bushen, a Chinese‐patented drug with the function of relieving fatigue and delaying ageing, has been proven effective for extenuating skin senescence. To investigate the potential mechanism, senescence‐accelerated mouse prone 6 (SAMP6) was intragastrically administered with Bazi Bushen for 9 we...

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Autores principales: Xu, Zhe, Gong, Boyang, Li, Zhaodong, Wang, Ying, Zhao, Zeyu, Xie, Lulu, Peng, Yanfei, Zhao, Shuwu, Zhou, Huifang, Bian, Yuhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10494291/
https://www.ncbi.nlm.nih.gov/pubmed/37614114
http://dx.doi.org/10.1111/jcmm.17833
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author Xu, Zhe
Gong, Boyang
Li, Zhaodong
Wang, Ying
Zhao, Zeyu
Xie, Lulu
Peng, Yanfei
Zhao, Shuwu
Zhou, Huifang
Bian, Yuhong
author_facet Xu, Zhe
Gong, Boyang
Li, Zhaodong
Wang, Ying
Zhao, Zeyu
Xie, Lulu
Peng, Yanfei
Zhao, Shuwu
Zhou, Huifang
Bian, Yuhong
author_sort Xu, Zhe
collection PubMed
description Bazi Bushen, a Chinese‐patented drug with the function of relieving fatigue and delaying ageing, has been proven effective for extenuating skin senescence. To investigate the potential mechanism, senescence‐accelerated mouse prone 6 (SAMP6) was intragastrically administered with Bazi Bushen for 9 weeks to induce skin homeostasis. Skin homeostasis is important in mitigating skin senescence, and it is related to many factors such as oxidative stress, SASP, apoptosis, autophagy and stem cell. In our study, skin damage in SAMP6 mice was observed using HE, Masson and SA‐β‐gal staining. The content of hydroxyproline and the activities of SOD, MDA, GSH‐PX and T‐AOC in the skin were measured using commercial assay kits. The level of SASP factors (IL‐6, IL‐1β, TNF‐α, MMP2 and MMP9) in skin were measured using ELISA kits. The protein expressions of p16, p21, p53, Bax, Bcl‐2, Cleaved caspase‐3, LC3, p62, Beclin1, OCT4, SOX2 and NANOG were measured by western blotting. The expression of ITGA6 and COL17A1 was measured by immunofluorescence staining and western blotting. Our findings demonstrated that Bazi Bushen alleviated skin senescence by orchestrating skin homeostasis, reducing the level of oxidative stress and the expression of SASP, regulating the balance of apoptosis and autophagy and enhancing the protein expressions of ITGA6 and COL17A1 to improve skin structure in SAMP6 mice. This study indicated that Bazi Bushen could serve as a potential therapy for alleviating skin senescence.
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spelling pubmed-104942912023-09-12 Bazi Bushen alleviates skin senescence by orchestrating skin homeostasis in SAMP6 mice Xu, Zhe Gong, Boyang Li, Zhaodong Wang, Ying Zhao, Zeyu Xie, Lulu Peng, Yanfei Zhao, Shuwu Zhou, Huifang Bian, Yuhong J Cell Mol Med Original Articles Bazi Bushen, a Chinese‐patented drug with the function of relieving fatigue and delaying ageing, has been proven effective for extenuating skin senescence. To investigate the potential mechanism, senescence‐accelerated mouse prone 6 (SAMP6) was intragastrically administered with Bazi Bushen for 9 weeks to induce skin homeostasis. Skin homeostasis is important in mitigating skin senescence, and it is related to many factors such as oxidative stress, SASP, apoptosis, autophagy and stem cell. In our study, skin damage in SAMP6 mice was observed using HE, Masson and SA‐β‐gal staining. The content of hydroxyproline and the activities of SOD, MDA, GSH‐PX and T‐AOC in the skin were measured using commercial assay kits. The level of SASP factors (IL‐6, IL‐1β, TNF‐α, MMP2 and MMP9) in skin were measured using ELISA kits. The protein expressions of p16, p21, p53, Bax, Bcl‐2, Cleaved caspase‐3, LC3, p62, Beclin1, OCT4, SOX2 and NANOG were measured by western blotting. The expression of ITGA6 and COL17A1 was measured by immunofluorescence staining and western blotting. Our findings demonstrated that Bazi Bushen alleviated skin senescence by orchestrating skin homeostasis, reducing the level of oxidative stress and the expression of SASP, regulating the balance of apoptosis and autophagy and enhancing the protein expressions of ITGA6 and COL17A1 to improve skin structure in SAMP6 mice. This study indicated that Bazi Bushen could serve as a potential therapy for alleviating skin senescence. John Wiley and Sons Inc. 2023-08-23 /pmc/articles/PMC10494291/ /pubmed/37614114 http://dx.doi.org/10.1111/jcmm.17833 Text en © 2023 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Xu, Zhe
Gong, Boyang
Li, Zhaodong
Wang, Ying
Zhao, Zeyu
Xie, Lulu
Peng, Yanfei
Zhao, Shuwu
Zhou, Huifang
Bian, Yuhong
Bazi Bushen alleviates skin senescence by orchestrating skin homeostasis in SAMP6 mice
title Bazi Bushen alleviates skin senescence by orchestrating skin homeostasis in SAMP6 mice
title_full Bazi Bushen alleviates skin senescence by orchestrating skin homeostasis in SAMP6 mice
title_fullStr Bazi Bushen alleviates skin senescence by orchestrating skin homeostasis in SAMP6 mice
title_full_unstemmed Bazi Bushen alleviates skin senescence by orchestrating skin homeostasis in SAMP6 mice
title_short Bazi Bushen alleviates skin senescence by orchestrating skin homeostasis in SAMP6 mice
title_sort bazi bushen alleviates skin senescence by orchestrating skin homeostasis in samp6 mice
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10494291/
https://www.ncbi.nlm.nih.gov/pubmed/37614114
http://dx.doi.org/10.1111/jcmm.17833
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