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Effects of antimicrobial peptide and tributyrin on fecal microflora and blood indices of female calves

This study evaluated the effects of antimicrobial peptide (AMP) and tributyrin (TB) on dairy calves in terms of growth performance, immunity, oxidative stress, and intestinal microflora. A total of 40 female calves were divided into four treatment groups (n = 10): basal diet +0.015% essential oil, b...

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Autores principales: Gao, Junling, Dong, Jianan, Sun, Zhe, Wang, Tao, Guan, Yanling, Sun, Yue, Qin, Guixin, Zhang, Xuefeng, Zhen, Yuguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10494653/
https://www.ncbi.nlm.nih.gov/pubmed/37701190
http://dx.doi.org/10.1002/fsn3.3483
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author Gao, Junling
Dong, Jianan
Sun, Zhe
Wang, Tao
Guan, Yanling
Sun, Yue
Qin, Guixin
Zhang, Xuefeng
Zhen, Yuguo
author_facet Gao, Junling
Dong, Jianan
Sun, Zhe
Wang, Tao
Guan, Yanling
Sun, Yue
Qin, Guixin
Zhang, Xuefeng
Zhen, Yuguo
author_sort Gao, Junling
collection PubMed
description This study evaluated the effects of antimicrobial peptide (AMP) and tributyrin (TB) on dairy calves in terms of growth performance, immunity, oxidative stress, and intestinal microflora. A total of 40 female calves were divided into four treatment groups (n = 10): basal diet +0.015% essential oil, basal diet +0.03% AMP, basal diet +0.15% TB, and basal diet +0.03% AMP + 0.15% TB. AMP and TB supplementation increased the average daily gain (ADG) and weaning weight, while reducing diarrhea occurrence. Additionally, AMP and TB supplementation reduced the levels of reactive oxygen species (ROS) and malonaldehyde (MDA), while increasing superoxide dismutase (SOD) levels and serum immunoglobulin M (IgM) levels. However, the combined use of AMP and TB did not significantly affect the average daily feed intake, ADG, weaning weight, or diarrhea incidence but decreased ROS levels, while increasing SOD levels as well as MDA and IgM levels. Moreover, AMP and TG supplementation increased the relative abundance of several beneficial fiber‐ and mucin‐degrading bacteria in the gut, in contrast to combined AMP and TB supplementation. The 16S rRNA results showed that AMP supplementation significantly increased the relative abundance of Rikenellaceae_RC9_gut_group, Ruminococcaceae_UCG‐014 and [Eubacterium]_coprostanoligenes group (p < .01), and significantly decreased the relative abundance of Ruminococcaceae_UCG‐005 and Christensenellaceae_R‐7_group (p < .01). The TB supplementation significantly increased the abundances of Rikenellaceae_RC9_gut_group and Ruminococcaceae_UCG‐005 (p < .01), and significantly decreased the relative abundances of Ruminococcaceae_UCG‐014, [Eubacterium]_coprostanoligenes group and Christensenellaceae_R‐7_group (p < .01). The combined use of AMP and TB significantly increased the relative abundance of Rikenellaceae_RC9_gut_group and Bacteroides (p < .01), and significantly decreased the relative abundance of Ruminococcaceae_UCG‐014, [Eubacterium]_coprostanoligenes group and Christensenellaceae_R‐7_group (p < .01). In summary, diets supplemented with either AMP or TB improved the intestinal microflora, growth performance, and health of weaned calves, but combined use was detrimental to calf performance.
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spelling pubmed-104946532023-09-12 Effects of antimicrobial peptide and tributyrin on fecal microflora and blood indices of female calves Gao, Junling Dong, Jianan Sun, Zhe Wang, Tao Guan, Yanling Sun, Yue Qin, Guixin Zhang, Xuefeng Zhen, Yuguo Food Sci Nutr Original Articles This study evaluated the effects of antimicrobial peptide (AMP) and tributyrin (TB) on dairy calves in terms of growth performance, immunity, oxidative stress, and intestinal microflora. A total of 40 female calves were divided into four treatment groups (n = 10): basal diet +0.015% essential oil, basal diet +0.03% AMP, basal diet +0.15% TB, and basal diet +0.03% AMP + 0.15% TB. AMP and TB supplementation increased the average daily gain (ADG) and weaning weight, while reducing diarrhea occurrence. Additionally, AMP and TB supplementation reduced the levels of reactive oxygen species (ROS) and malonaldehyde (MDA), while increasing superoxide dismutase (SOD) levels and serum immunoglobulin M (IgM) levels. However, the combined use of AMP and TB did not significantly affect the average daily feed intake, ADG, weaning weight, or diarrhea incidence but decreased ROS levels, while increasing SOD levels as well as MDA and IgM levels. Moreover, AMP and TG supplementation increased the relative abundance of several beneficial fiber‐ and mucin‐degrading bacteria in the gut, in contrast to combined AMP and TB supplementation. The 16S rRNA results showed that AMP supplementation significantly increased the relative abundance of Rikenellaceae_RC9_gut_group, Ruminococcaceae_UCG‐014 and [Eubacterium]_coprostanoligenes group (p < .01), and significantly decreased the relative abundance of Ruminococcaceae_UCG‐005 and Christensenellaceae_R‐7_group (p < .01). The TB supplementation significantly increased the abundances of Rikenellaceae_RC9_gut_group and Ruminococcaceae_UCG‐005 (p < .01), and significantly decreased the relative abundances of Ruminococcaceae_UCG‐014, [Eubacterium]_coprostanoligenes group and Christensenellaceae_R‐7_group (p < .01). The combined use of AMP and TB significantly increased the relative abundance of Rikenellaceae_RC9_gut_group and Bacteroides (p < .01), and significantly decreased the relative abundance of Ruminococcaceae_UCG‐014, [Eubacterium]_coprostanoligenes group and Christensenellaceae_R‐7_group (p < .01). In summary, diets supplemented with either AMP or TB improved the intestinal microflora, growth performance, and health of weaned calves, but combined use was detrimental to calf performance. John Wiley and Sons Inc. 2023-06-12 /pmc/articles/PMC10494653/ /pubmed/37701190 http://dx.doi.org/10.1002/fsn3.3483 Text en © 2023 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Gao, Junling
Dong, Jianan
Sun, Zhe
Wang, Tao
Guan, Yanling
Sun, Yue
Qin, Guixin
Zhang, Xuefeng
Zhen, Yuguo
Effects of antimicrobial peptide and tributyrin on fecal microflora and blood indices of female calves
title Effects of antimicrobial peptide and tributyrin on fecal microflora and blood indices of female calves
title_full Effects of antimicrobial peptide and tributyrin on fecal microflora and blood indices of female calves
title_fullStr Effects of antimicrobial peptide and tributyrin on fecal microflora and blood indices of female calves
title_full_unstemmed Effects of antimicrobial peptide and tributyrin on fecal microflora and blood indices of female calves
title_short Effects of antimicrobial peptide and tributyrin on fecal microflora and blood indices of female calves
title_sort effects of antimicrobial peptide and tributyrin on fecal microflora and blood indices of female calves
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10494653/
https://www.ncbi.nlm.nih.gov/pubmed/37701190
http://dx.doi.org/10.1002/fsn3.3483
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