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Legume-nodulating rhizobia are widespread in soils and plants across the island of O‘ahu, Hawai‘i

Legumes and their interaction with rhizobia represent one of the most well-characterized symbioses that are widespread across both natural and agricultural environments. However, larger distribution patterns and host associations on isolated Pacific islands with many native and introduced hosts have...

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Autores principales: Abe, Jonathan N. A., Dhungana, Ishwora, Nguyen, Nhu H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495000/
https://www.ncbi.nlm.nih.gov/pubmed/37695782
http://dx.doi.org/10.1371/journal.pone.0291250
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author Abe, Jonathan N. A.
Dhungana, Ishwora
Nguyen, Nhu H.
author_facet Abe, Jonathan N. A.
Dhungana, Ishwora
Nguyen, Nhu H.
author_sort Abe, Jonathan N. A.
collection PubMed
description Legumes and their interaction with rhizobia represent one of the most well-characterized symbioses that are widespread across both natural and agricultural environments. However, larger distribution patterns and host associations on isolated Pacific islands with many native and introduced hosts have not been well-documented. Here, we used molecular and culturing techniques to characterize rhizobia from soils and 24 native and introduced legume species on the island of O’ahu, Hawai‘i. We chose two of these isolates to inoculate an endemic legume tree, Erythina sandwicensis to measure nodulation potentials and host benefits. We found that all rhizobia genera can be found in the soil, where only Cupriavidus was found at all sites, although at lower abundance relative to other more common genera such as Rhizobium (and close relatives), Bradyzhizobium, and Devosia. Bradyrhizobium was the most common nodulator of legumes, where the strain Bradyrhizobium sp. strain JA1 is a generalist capable of forming nodules on nine different host species, including two native species. In greenhouse nursery inoculations, the two different Bradyrhizobium strains successfully nodulate the endemic E. sandwicensis; both strains equally and significantly increased seedling biomass in nursery inoculations. Overall, this work provides a molecular-based framework in which to study potential native and introduced rhizobia on one of the most isolated archipelagos on the planet.
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spelling pubmed-104950002023-09-12 Legume-nodulating rhizobia are widespread in soils and plants across the island of O‘ahu, Hawai‘i Abe, Jonathan N. A. Dhungana, Ishwora Nguyen, Nhu H. PLoS One Research Article Legumes and their interaction with rhizobia represent one of the most well-characterized symbioses that are widespread across both natural and agricultural environments. However, larger distribution patterns and host associations on isolated Pacific islands with many native and introduced hosts have not been well-documented. Here, we used molecular and culturing techniques to characterize rhizobia from soils and 24 native and introduced legume species on the island of O’ahu, Hawai‘i. We chose two of these isolates to inoculate an endemic legume tree, Erythina sandwicensis to measure nodulation potentials and host benefits. We found that all rhizobia genera can be found in the soil, where only Cupriavidus was found at all sites, although at lower abundance relative to other more common genera such as Rhizobium (and close relatives), Bradyzhizobium, and Devosia. Bradyrhizobium was the most common nodulator of legumes, where the strain Bradyrhizobium sp. strain JA1 is a generalist capable of forming nodules on nine different host species, including two native species. In greenhouse nursery inoculations, the two different Bradyrhizobium strains successfully nodulate the endemic E. sandwicensis; both strains equally and significantly increased seedling biomass in nursery inoculations. Overall, this work provides a molecular-based framework in which to study potential native and introduced rhizobia on one of the most isolated archipelagos on the planet. Public Library of Science 2023-09-11 /pmc/articles/PMC10495000/ /pubmed/37695782 http://dx.doi.org/10.1371/journal.pone.0291250 Text en © 2023 Abe et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Abe, Jonathan N. A.
Dhungana, Ishwora
Nguyen, Nhu H.
Legume-nodulating rhizobia are widespread in soils and plants across the island of O‘ahu, Hawai‘i
title Legume-nodulating rhizobia are widespread in soils and plants across the island of O‘ahu, Hawai‘i
title_full Legume-nodulating rhizobia are widespread in soils and plants across the island of O‘ahu, Hawai‘i
title_fullStr Legume-nodulating rhizobia are widespread in soils and plants across the island of O‘ahu, Hawai‘i
title_full_unstemmed Legume-nodulating rhizobia are widespread in soils and plants across the island of O‘ahu, Hawai‘i
title_short Legume-nodulating rhizobia are widespread in soils and plants across the island of O‘ahu, Hawai‘i
title_sort legume-nodulating rhizobia are widespread in soils and plants across the island of o‘ahu, hawai‘i
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495000/
https://www.ncbi.nlm.nih.gov/pubmed/37695782
http://dx.doi.org/10.1371/journal.pone.0291250
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