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Functional knockout of long non-coding RNAs with genome editing

An effective loss-of-function study is necessary to investigate the biological function of long non-coding RNA (lncRNA). Various approaches are available, including RNA silencing, antisense oligos, and CRISPR-based genome editing. CRISPR-based genome editing is the most widely used for inactivating...

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Autores principales: Lyu, Qing Rex, Zhang, Shikuan, Zhang, Zhe, Tang, Zhiyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495571/
https://www.ncbi.nlm.nih.gov/pubmed/37705609
http://dx.doi.org/10.3389/fgene.2023.1242129
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author Lyu, Qing Rex
Zhang, Shikuan
Zhang, Zhe
Tang, Zhiyu
author_facet Lyu, Qing Rex
Zhang, Shikuan
Zhang, Zhe
Tang, Zhiyu
author_sort Lyu, Qing Rex
collection PubMed
description An effective loss-of-function study is necessary to investigate the biological function of long non-coding RNA (lncRNA). Various approaches are available, including RNA silencing, antisense oligos, and CRISPR-based genome editing. CRISPR-based genome editing is the most widely used for inactivating lncRNA function at the genomic level. Knocking out the lncRNA function can be achieved by removing the promoter and the first exon (PE1), introducing pre-termination poly(A) signals, or deleting the entire locus, unlike frameshift strategies used for messenger RNA (mRNA). However, the intricate genomic interplay between lncRNA and neighbor genes makes it challenging to interpret lncRNA function accurately. This article discusses the advantages and disadvantages of each lncRNA knockout method and envisions the potential future directions to facilitate lncRNA functional study.
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spelling pubmed-104955712023-09-13 Functional knockout of long non-coding RNAs with genome editing Lyu, Qing Rex Zhang, Shikuan Zhang, Zhe Tang, Zhiyu Front Genet Genetics An effective loss-of-function study is necessary to investigate the biological function of long non-coding RNA (lncRNA). Various approaches are available, including RNA silencing, antisense oligos, and CRISPR-based genome editing. CRISPR-based genome editing is the most widely used for inactivating lncRNA function at the genomic level. Knocking out the lncRNA function can be achieved by removing the promoter and the first exon (PE1), introducing pre-termination poly(A) signals, or deleting the entire locus, unlike frameshift strategies used for messenger RNA (mRNA). However, the intricate genomic interplay between lncRNA and neighbor genes makes it challenging to interpret lncRNA function accurately. This article discusses the advantages and disadvantages of each lncRNA knockout method and envisions the potential future directions to facilitate lncRNA functional study. Frontiers Media S.A. 2023-08-29 /pmc/articles/PMC10495571/ /pubmed/37705609 http://dx.doi.org/10.3389/fgene.2023.1242129 Text en Copyright © 2023 Lyu, Zhang, Zhang and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Lyu, Qing Rex
Zhang, Shikuan
Zhang, Zhe
Tang, Zhiyu
Functional knockout of long non-coding RNAs with genome editing
title Functional knockout of long non-coding RNAs with genome editing
title_full Functional knockout of long non-coding RNAs with genome editing
title_fullStr Functional knockout of long non-coding RNAs with genome editing
title_full_unstemmed Functional knockout of long non-coding RNAs with genome editing
title_short Functional knockout of long non-coding RNAs with genome editing
title_sort functional knockout of long non-coding rnas with genome editing
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495571/
https://www.ncbi.nlm.nih.gov/pubmed/37705609
http://dx.doi.org/10.3389/fgene.2023.1242129
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