Calculating RNA degradation rates using large-scale normalization in mouse embryonic stem cells

Data normalization is critical to the process of estimating RNA degradation by analyzing RNA levels when transcription is blocked. Here, we present a protocol for measuring mRNA degradation rates, optimized for mouse embryonic stem cells, using α-amanitin inhibitor. We describe steps for a time cour...

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Detalles Bibliográficos
Autores principales: Viegas, Juliane Oliveira, Fishman, Lior, Meshorer, Eran, Rabani, Michal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495639/
https://www.ncbi.nlm.nih.gov/pubmed/37656628
http://dx.doi.org/10.1016/j.xpro.2023.102534

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