Protocol to image and quantify nuclear pore complexes using high-resolution laser scanning confocal microscopy

Nuclear pore complexes are pathways for nuclear-cytoplasmic communication that participate in chromatin organization. Here, we present a protocol to image and quantify the number of nuclear pore complexes in cells. We describe steps for cell plating and culture, immunofluorescence detection, and con...

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Detalles Bibliográficos
Autores principales: Mich-Basso, Jocelyn D., Kühn, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495640/
https://www.ncbi.nlm.nih.gov/pubmed/37651236
http://dx.doi.org/10.1016/j.xpro.2023.102552

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495640/
https://www.ncbi.nlm.nih.gov/pubmed/37651236
http://dx.doi.org/10.1016/j.xpro.2023.102552

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