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Protocol to image and quantify nuclear pore complexes using high-resolution laser scanning confocal microscopy
Nuclear pore complexes are pathways for nuclear-cytoplasmic communication that participate in chromatin organization. Here, we present a protocol to image and quantify the number of nuclear pore complexes in cells. We describe steps for cell plating and culture, immunofluorescence detection, and con...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495640/ https://www.ncbi.nlm.nih.gov/pubmed/37651236 http://dx.doi.org/10.1016/j.xpro.2023.102552 |