Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis
BACKGROUND: Chromatin immunoprecipitation (ChIP) analysis revealed that the FBXW7 gene and the long non-coding RNA (LINC01588) are potential candidates in epithelial ovarian cancer (EOC) pathogenesis. However, their exact role in EOC is not yet known. Thus, the present study sheds light on the impac...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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West Asia Organization for Cancer Prevention
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495899/ https://www.ncbi.nlm.nih.gov/pubmed/37247277 http://dx.doi.org/10.31557/APJCP.2023.24.5.1583 |
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author | Al Hinai, Meerah Malgundkar, Shika Hanif Gupta, Ishita Lakhtakia, Ritu Al Kalbani, Moza Burney, Ikram Al Moundhri, Mansour Okamoto, Aikou Tamimi, Yahya |
author_facet | Al Hinai, Meerah Malgundkar, Shika Hanif Gupta, Ishita Lakhtakia, Ritu Al Kalbani, Moza Burney, Ikram Al Moundhri, Mansour Okamoto, Aikou Tamimi, Yahya |
author_sort | Al Hinai, Meerah |
collection | PubMed |
description | BACKGROUND: Chromatin immunoprecipitation (ChIP) analysis revealed that the FBXW7 gene and the long non-coding RNA (LINC01588) are potential candidates in epithelial ovarian cancer (EOC) pathogenesis. However, their exact role in EOC is not yet known. Thus, the present study sheds light on the impact of the mutations/ methylation status of the FBXW7 gene. MATERIALS AND METHODS: We used public databases to assess the correlation between mutations/ methylation status and the FBXW7 expression. Furthermore, we performed Pearson’s correlation analysis between the FBXW7 gene and LINC01588. We performed gene panel exome sequencing and Methylation-specific PCR (MSP) in HOSE 6-3, MCAS, OVSAHO, and eight EOC patients’ samples to validate the bioinformatics results. RESULTS: The FBXW7 gene was less expressed in EOC, particularly in stages III and IV, compared to healthy tissues. Furthermore, bioinformatics analysis, gene panel exome sequencing, and MSP revealed that the FBXW7 gene is neither mutated nor methylated in EOC cell lines and tissues, suggesting alternative mechanisms for FBXW7 gene regulation. Interestingly, Pearson’s correlation analysis showed an inverse, significant correlation between the FBXW7 gene and LINC01588 expression, suggesting a potential regulatory role of LINC01588. CONCLUSION: Neither mutations nor methylation is the causative mechanism for the FBXW7 downregulation in EOC, suggesting alternative means involving the lncRNA LINC01588. |
format | Online Article Text |
id | pubmed-10495899 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | West Asia Organization for Cancer Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-104958992023-09-13 Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis Al Hinai, Meerah Malgundkar, Shika Hanif Gupta, Ishita Lakhtakia, Ritu Al Kalbani, Moza Burney, Ikram Al Moundhri, Mansour Okamoto, Aikou Tamimi, Yahya Asian Pac J Cancer Prev Research Article BACKGROUND: Chromatin immunoprecipitation (ChIP) analysis revealed that the FBXW7 gene and the long non-coding RNA (LINC01588) are potential candidates in epithelial ovarian cancer (EOC) pathogenesis. However, their exact role in EOC is not yet known. Thus, the present study sheds light on the impact of the mutations/ methylation status of the FBXW7 gene. MATERIALS AND METHODS: We used public databases to assess the correlation between mutations/ methylation status and the FBXW7 expression. Furthermore, we performed Pearson’s correlation analysis between the FBXW7 gene and LINC01588. We performed gene panel exome sequencing and Methylation-specific PCR (MSP) in HOSE 6-3, MCAS, OVSAHO, and eight EOC patients’ samples to validate the bioinformatics results. RESULTS: The FBXW7 gene was less expressed in EOC, particularly in stages III and IV, compared to healthy tissues. Furthermore, bioinformatics analysis, gene panel exome sequencing, and MSP revealed that the FBXW7 gene is neither mutated nor methylated in EOC cell lines and tissues, suggesting alternative mechanisms for FBXW7 gene regulation. Interestingly, Pearson’s correlation analysis showed an inverse, significant correlation between the FBXW7 gene and LINC01588 expression, suggesting a potential regulatory role of LINC01588. CONCLUSION: Neither mutations nor methylation is the causative mechanism for the FBXW7 downregulation in EOC, suggesting alternative means involving the lncRNA LINC01588. West Asia Organization for Cancer Prevention 2023 /pmc/articles/PMC10495899/ /pubmed/37247277 http://dx.doi.org/10.31557/APJCP.2023.24.5.1583 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. (https://creativecommons.org/licenses/by-nc/4.0/) |
spellingShingle | Research Article Al Hinai, Meerah Malgundkar, Shika Hanif Gupta, Ishita Lakhtakia, Ritu Al Kalbani, Moza Burney, Ikram Al Moundhri, Mansour Okamoto, Aikou Tamimi, Yahya Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis |
title | Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis |
title_full | Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis |
title_fullStr | Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis |
title_full_unstemmed | Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis |
title_short | Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis |
title_sort | epigenetic status of fbxw7 gene and its role in ovarian cancer pathogenesis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495899/ https://www.ncbi.nlm.nih.gov/pubmed/37247277 http://dx.doi.org/10.31557/APJCP.2023.24.5.1583 |
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