Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis

BACKGROUND: Chromatin immunoprecipitation (ChIP) analysis revealed that the FBXW7 gene and the long non-coding RNA (LINC01588) are potential candidates in epithelial ovarian cancer (EOC) pathogenesis. However, their exact role in EOC is not yet known. Thus, the present study sheds light on the impac...

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Autores principales: Al Hinai, Meerah, Malgundkar, Shika Hanif, Gupta, Ishita, Lakhtakia, Ritu, Al Kalbani, Moza, Burney, Ikram, Al Moundhri, Mansour, Okamoto, Aikou, Tamimi, Yahya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495899/
https://www.ncbi.nlm.nih.gov/pubmed/37247277
http://dx.doi.org/10.31557/APJCP.2023.24.5.1583
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author Al Hinai, Meerah
Malgundkar, Shika Hanif
Gupta, Ishita
Lakhtakia, Ritu
Al Kalbani, Moza
Burney, Ikram
Al Moundhri, Mansour
Okamoto, Aikou
Tamimi, Yahya
author_facet Al Hinai, Meerah
Malgundkar, Shika Hanif
Gupta, Ishita
Lakhtakia, Ritu
Al Kalbani, Moza
Burney, Ikram
Al Moundhri, Mansour
Okamoto, Aikou
Tamimi, Yahya
author_sort Al Hinai, Meerah
collection PubMed
description BACKGROUND: Chromatin immunoprecipitation (ChIP) analysis revealed that the FBXW7 gene and the long non-coding RNA (LINC01588) are potential candidates in epithelial ovarian cancer (EOC) pathogenesis. However, their exact role in EOC is not yet known. Thus, the present study sheds light on the impact of the mutations/ methylation status of the FBXW7 gene. MATERIALS AND METHODS: We used public databases to assess the correlation between mutations/ methylation status and the FBXW7 expression. Furthermore, we performed Pearson’s correlation analysis between the FBXW7 gene and LINC01588. We performed gene panel exome sequencing and Methylation-specific PCR (MSP) in HOSE 6-3, MCAS, OVSAHO, and eight EOC patients’ samples to validate the bioinformatics results. RESULTS: The FBXW7 gene was less expressed in EOC, particularly in stages III and IV, compared to healthy tissues. Furthermore, bioinformatics analysis, gene panel exome sequencing, and MSP revealed that the FBXW7 gene is neither mutated nor methylated in EOC cell lines and tissues, suggesting alternative mechanisms for FBXW7 gene regulation. Interestingly, Pearson’s correlation analysis showed an inverse, significant correlation between the FBXW7 gene and LINC01588 expression, suggesting a potential regulatory role of LINC01588. CONCLUSION: Neither mutations nor methylation is the causative mechanism for the FBXW7 downregulation in EOC, suggesting alternative means involving the lncRNA LINC01588.
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spelling pubmed-104958992023-09-13 Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis Al Hinai, Meerah Malgundkar, Shika Hanif Gupta, Ishita Lakhtakia, Ritu Al Kalbani, Moza Burney, Ikram Al Moundhri, Mansour Okamoto, Aikou Tamimi, Yahya Asian Pac J Cancer Prev Research Article BACKGROUND: Chromatin immunoprecipitation (ChIP) analysis revealed that the FBXW7 gene and the long non-coding RNA (LINC01588) are potential candidates in epithelial ovarian cancer (EOC) pathogenesis. However, their exact role in EOC is not yet known. Thus, the present study sheds light on the impact of the mutations/ methylation status of the FBXW7 gene. MATERIALS AND METHODS: We used public databases to assess the correlation between mutations/ methylation status and the FBXW7 expression. Furthermore, we performed Pearson’s correlation analysis between the FBXW7 gene and LINC01588. We performed gene panel exome sequencing and Methylation-specific PCR (MSP) in HOSE 6-3, MCAS, OVSAHO, and eight EOC patients’ samples to validate the bioinformatics results. RESULTS: The FBXW7 gene was less expressed in EOC, particularly in stages III and IV, compared to healthy tissues. Furthermore, bioinformatics analysis, gene panel exome sequencing, and MSP revealed that the FBXW7 gene is neither mutated nor methylated in EOC cell lines and tissues, suggesting alternative mechanisms for FBXW7 gene regulation. Interestingly, Pearson’s correlation analysis showed an inverse, significant correlation between the FBXW7 gene and LINC01588 expression, suggesting a potential regulatory role of LINC01588. CONCLUSION: Neither mutations nor methylation is the causative mechanism for the FBXW7 downregulation in EOC, suggesting alternative means involving the lncRNA LINC01588. West Asia Organization for Cancer Prevention 2023 /pmc/articles/PMC10495899/ /pubmed/37247277 http://dx.doi.org/10.31557/APJCP.2023.24.5.1583 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. (https://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle Research Article
Al Hinai, Meerah
Malgundkar, Shika Hanif
Gupta, Ishita
Lakhtakia, Ritu
Al Kalbani, Moza
Burney, Ikram
Al Moundhri, Mansour
Okamoto, Aikou
Tamimi, Yahya
Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis
title Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis
title_full Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis
title_fullStr Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis
title_full_unstemmed Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis
title_short Epigenetic Status of FBXW7 Gene and Its Role in Ovarian Cancer Pathogenesis
title_sort epigenetic status of fbxw7 gene and its role in ovarian cancer pathogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495899/
https://www.ncbi.nlm.nih.gov/pubmed/37247277
http://dx.doi.org/10.31557/APJCP.2023.24.5.1583
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