Cargando…
Blue‐green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers
Infection of Arabidopsis with avirulent Pseudomonas syringae and exposure to nitrogen dioxide (NO(2)) both trigger hypersensitive cell death (HCD) that is characterized by the emission of bright blue‐green (BG) autofluorescence under UV illumination. The aim of our current work was to identify the B...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10496137/ https://www.ncbi.nlm.nih.gov/pubmed/37705693 http://dx.doi.org/10.1002/pld3.531 |
_version_ | 1785105045336883200 |
---|---|
author | Kanawati, Basem Bertic, Marko Moritz, Franco Habermann, Felix Zimmer, Ina Mackey, David Schmitt‐Kopplin, Philippe Schnitzler, Jörg‐Peter Durner, Jörg Gaupels, Frank |
author_facet | Kanawati, Basem Bertic, Marko Moritz, Franco Habermann, Felix Zimmer, Ina Mackey, David Schmitt‐Kopplin, Philippe Schnitzler, Jörg‐Peter Durner, Jörg Gaupels, Frank |
author_sort | Kanawati, Basem |
collection | PubMed |
description | Infection of Arabidopsis with avirulent Pseudomonas syringae and exposure to nitrogen dioxide (NO(2)) both trigger hypersensitive cell death (HCD) that is characterized by the emission of bright blue‐green (BG) autofluorescence under UV illumination. The aim of our current work was to identify the BG fluorescent molecules and scrutinize their biosynthesis, localization, and functions during the HCD. Compared with wild‐type (WT) plants, the phenylpropanoid‐deficient mutant fah1 developed normal HCD except for the absence of BG fluorescence. Ultrahigh resolution metabolomics combined with mass difference network analysis revealed that WT but not fah1 plants rapidly accumulate dehydrodimers of sinapic acid, sinapoylmalate, 5‐hydroxyferulic acid, and 5‐hydroxyferuloylmalate during the HCD. FAH1‐dependent BG fluorescence appeared exclusively within dying cells of the upper epidermis as detected by microscopy. Saponification released dehydrodimers from cell wall polymers of WT but not fah1 plants. Collectively, our data suggest that HCD induction leads to the formation of free BG fluorescent dehydrodimers from monomeric sinapates and 5‐hydroxyferulates. The formed dehydrodimers move from upper epidermis cells into the apoplast where they esterify cell wall polymers. Possible functions of phenylpropanoid dehydrodimers are discussed. |
format | Online Article Text |
id | pubmed-10496137 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104961372023-09-13 Blue‐green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers Kanawati, Basem Bertic, Marko Moritz, Franco Habermann, Felix Zimmer, Ina Mackey, David Schmitt‐Kopplin, Philippe Schnitzler, Jörg‐Peter Durner, Jörg Gaupels, Frank Plant Direct Research Articles Infection of Arabidopsis with avirulent Pseudomonas syringae and exposure to nitrogen dioxide (NO(2)) both trigger hypersensitive cell death (HCD) that is characterized by the emission of bright blue‐green (BG) autofluorescence under UV illumination. The aim of our current work was to identify the BG fluorescent molecules and scrutinize their biosynthesis, localization, and functions during the HCD. Compared with wild‐type (WT) plants, the phenylpropanoid‐deficient mutant fah1 developed normal HCD except for the absence of BG fluorescence. Ultrahigh resolution metabolomics combined with mass difference network analysis revealed that WT but not fah1 plants rapidly accumulate dehydrodimers of sinapic acid, sinapoylmalate, 5‐hydroxyferulic acid, and 5‐hydroxyferuloylmalate during the HCD. FAH1‐dependent BG fluorescence appeared exclusively within dying cells of the upper epidermis as detected by microscopy. Saponification released dehydrodimers from cell wall polymers of WT but not fah1 plants. Collectively, our data suggest that HCD induction leads to the formation of free BG fluorescent dehydrodimers from monomeric sinapates and 5‐hydroxyferulates. The formed dehydrodimers move from upper epidermis cells into the apoplast where they esterify cell wall polymers. Possible functions of phenylpropanoid dehydrodimers are discussed. John Wiley and Sons Inc. 2023-09-12 /pmc/articles/PMC10496137/ /pubmed/37705693 http://dx.doi.org/10.1002/pld3.531 Text en © 2023 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Kanawati, Basem Bertic, Marko Moritz, Franco Habermann, Felix Zimmer, Ina Mackey, David Schmitt‐Kopplin, Philippe Schnitzler, Jörg‐Peter Durner, Jörg Gaupels, Frank Blue‐green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers |
title | Blue‐green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers |
title_full | Blue‐green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers |
title_fullStr | Blue‐green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers |
title_full_unstemmed | Blue‐green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers |
title_short | Blue‐green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers |
title_sort | blue‐green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10496137/ https://www.ncbi.nlm.nih.gov/pubmed/37705693 http://dx.doi.org/10.1002/pld3.531 |
work_keys_str_mv | AT kanawatibasem bluegreenfluorescenceduringhypersensitivecelldeatharisesfromphenylpropanoiddeydrodimers AT berticmarko bluegreenfluorescenceduringhypersensitivecelldeatharisesfromphenylpropanoiddeydrodimers AT moritzfranco bluegreenfluorescenceduringhypersensitivecelldeatharisesfromphenylpropanoiddeydrodimers AT habermannfelix bluegreenfluorescenceduringhypersensitivecelldeatharisesfromphenylpropanoiddeydrodimers AT zimmerina bluegreenfluorescenceduringhypersensitivecelldeatharisesfromphenylpropanoiddeydrodimers AT mackeydavid bluegreenfluorescenceduringhypersensitivecelldeatharisesfromphenylpropanoiddeydrodimers AT schmittkopplinphilippe bluegreenfluorescenceduringhypersensitivecelldeatharisesfromphenylpropanoiddeydrodimers AT schnitzlerjorgpeter bluegreenfluorescenceduringhypersensitivecelldeatharisesfromphenylpropanoiddeydrodimers AT durnerjorg bluegreenfluorescenceduringhypersensitivecelldeatharisesfromphenylpropanoiddeydrodimers AT gaupelsfrank bluegreenfluorescenceduringhypersensitivecelldeatharisesfromphenylpropanoiddeydrodimers |