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Vibrational spectroscopy identifies myocardial chemical modifications in heart failure with preserved ejection fraction

BACKGROUND: Vibrational spectroscopy can be a valuable tool to monitor the markers of cardiovascular diseases. In the present work, we explored the vibrational spectroscopy characteristics of the cardiac tissue in an experimental model of heart failure with preserved ejection fraction (HFpEF). The g...

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Autores principales: Pioppi, Leonardo, Parvan, Reza, Samrend, Alan, Silva, Gustavo Jose Justo, Paolantoni, Marco, Sassi, Paola, Cataliotti, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10496315/
https://www.ncbi.nlm.nih.gov/pubmed/37697391
http://dx.doi.org/10.1186/s12967-023-04465-0
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author Pioppi, Leonardo
Parvan, Reza
Samrend, Alan
Silva, Gustavo Jose Justo
Paolantoni, Marco
Sassi, Paola
Cataliotti, Alessandro
author_facet Pioppi, Leonardo
Parvan, Reza
Samrend, Alan
Silva, Gustavo Jose Justo
Paolantoni, Marco
Sassi, Paola
Cataliotti, Alessandro
author_sort Pioppi, Leonardo
collection PubMed
description BACKGROUND: Vibrational spectroscopy can be a valuable tool to monitor the markers of cardiovascular diseases. In the present work, we explored the vibrational spectroscopy characteristics of the cardiac tissue in an experimental model of heart failure with preserved ejection fraction (HFpEF). The goal was to detect early cardiac chemical modifications associated with the development of HFpEF. METHODS: We used the Fourier-transform infrared (FTIR) and Raman micro-spectroscopic techniques to provide complementary and objective tools for the histological assessment of heart tissues from an animal model of HFpEF. A new sampling technique was adopted (tissue print on a CaF(2) disk) to characterize the extracellular matrix. RESULTS: Several spectroscopic markers (lipids, carbohydrates, and glutamate bands) were recognized in the cardiac ventricles due to the comorbidities associated with the pathology, such as obesity and diabetes. Besides, abnormal collagen cross-linking and a decrease in tryptophan content were observed and related to the stiffening of ventricles and to the inflammatory state which is a favourable condition for HFpEF. CONCLUSIONS: By the analyses of tissues and tissue prints, FTIR and Raman techniques were shown to be highly sensitive and selective in detecting changes in the chemistry of the heart in experimental HFpEF and its related comorbidities. Vibrational spectroscopy is a new approach that can identify novel biomarkers for early detection of HFpEF. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12967-023-04465-0.
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spelling pubmed-104963152023-09-13 Vibrational spectroscopy identifies myocardial chemical modifications in heart failure with preserved ejection fraction Pioppi, Leonardo Parvan, Reza Samrend, Alan Silva, Gustavo Jose Justo Paolantoni, Marco Sassi, Paola Cataliotti, Alessandro J Transl Med Research BACKGROUND: Vibrational spectroscopy can be a valuable tool to monitor the markers of cardiovascular diseases. In the present work, we explored the vibrational spectroscopy characteristics of the cardiac tissue in an experimental model of heart failure with preserved ejection fraction (HFpEF). The goal was to detect early cardiac chemical modifications associated with the development of HFpEF. METHODS: We used the Fourier-transform infrared (FTIR) and Raman micro-spectroscopic techniques to provide complementary and objective tools for the histological assessment of heart tissues from an animal model of HFpEF. A new sampling technique was adopted (tissue print on a CaF(2) disk) to characterize the extracellular matrix. RESULTS: Several spectroscopic markers (lipids, carbohydrates, and glutamate bands) were recognized in the cardiac ventricles due to the comorbidities associated with the pathology, such as obesity and diabetes. Besides, abnormal collagen cross-linking and a decrease in tryptophan content were observed and related to the stiffening of ventricles and to the inflammatory state which is a favourable condition for HFpEF. CONCLUSIONS: By the analyses of tissues and tissue prints, FTIR and Raman techniques were shown to be highly sensitive and selective in detecting changes in the chemistry of the heart in experimental HFpEF and its related comorbidities. Vibrational spectroscopy is a new approach that can identify novel biomarkers for early detection of HFpEF. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12967-023-04465-0. BioMed Central 2023-09-11 /pmc/articles/PMC10496315/ /pubmed/37697391 http://dx.doi.org/10.1186/s12967-023-04465-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Pioppi, Leonardo
Parvan, Reza
Samrend, Alan
Silva, Gustavo Jose Justo
Paolantoni, Marco
Sassi, Paola
Cataliotti, Alessandro
Vibrational spectroscopy identifies myocardial chemical modifications in heart failure with preserved ejection fraction
title Vibrational spectroscopy identifies myocardial chemical modifications in heart failure with preserved ejection fraction
title_full Vibrational spectroscopy identifies myocardial chemical modifications in heart failure with preserved ejection fraction
title_fullStr Vibrational spectroscopy identifies myocardial chemical modifications in heart failure with preserved ejection fraction
title_full_unstemmed Vibrational spectroscopy identifies myocardial chemical modifications in heart failure with preserved ejection fraction
title_short Vibrational spectroscopy identifies myocardial chemical modifications in heart failure with preserved ejection fraction
title_sort vibrational spectroscopy identifies myocardial chemical modifications in heart failure with preserved ejection fraction
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10496315/
https://www.ncbi.nlm.nih.gov/pubmed/37697391
http://dx.doi.org/10.1186/s12967-023-04465-0
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