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Single-cell transcriptomics reveals zinc and copper ions homeostasis in epicardial adipose tissue of heart failure

Epicardial adipose tissue (EAT) is a unique visceral fat reservoir that shares an immune microenvironment without a distinct boundary with myocardium. Increasingly, visceral fat has been studied as a secondary immune organ, and EAT is no exception in this regard. Cellular subsets of EAT are associat...

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Autores principales: Zou, Rongjun, Zhang, Miao, Zou, Zengxiao, Shi, Wanting, Tan, Songtao, Wang, Chaojie, Xu, Wenliu, Jin, Jiaqi, Milton, Stephen, Chen, Yang, Wang, Ge, Fan, Xiaoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10496493/
https://www.ncbi.nlm.nih.gov/pubmed/37705737
http://dx.doi.org/10.7150/ijbs.82844
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author Zou, Rongjun
Zhang, Miao
Zou, Zengxiao
Shi, Wanting
Tan, Songtao
Wang, Chaojie
Xu, Wenliu
Jin, Jiaqi
Milton, Stephen
Chen, Yang
Wang, Ge
Fan, Xiaoping
author_facet Zou, Rongjun
Zhang, Miao
Zou, Zengxiao
Shi, Wanting
Tan, Songtao
Wang, Chaojie
Xu, Wenliu
Jin, Jiaqi
Milton, Stephen
Chen, Yang
Wang, Ge
Fan, Xiaoping
author_sort Zou, Rongjun
collection PubMed
description Epicardial adipose tissue (EAT) is a unique visceral fat reservoir that shares an immune microenvironment without a distinct boundary with myocardium. Increasingly, visceral fat has been studied as a secondary immune organ, and EAT is no exception in this regard. Cellular subsets of EAT are associated with disease development. In heart failure (HF) patients, however, the immune characteristics of EAT have rarely been studied, especially those non-immune cells related to the immune microenvironment. Herein, an analysis of seven EAT samples by single-cell RNA sequencing (scRNA-Seq) is presented here, including 1 neonate, 1 infant, 1 child, 2 adults with heart failure (Adults-HF) and 2 adult heart transplant donors as non-heart failure control (Adults-Non HF). Analysis of 51730 high-quality cells revealed eleven major cell types in EAT. For the first time, the pseudo-temporal reconstruction technique was employed to plot the cell trajectories of various major cell types (such as T lymphocytes, fibroblasts, endothelial cells, monocytes, and smooth muscle cells) in EAT across different developmental stages, achieving a single-cell resolution. The dynamic gene expression patterns of major cell types presented the immune characteristics of metabolism disorder of zinc and copper ions, and downregulated immune-related pathways in EAT of adult patients with HF. These data provide insights regarding HF immune dysregulation at the cellular level.
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spelling pubmed-104964932023-09-13 Single-cell transcriptomics reveals zinc and copper ions homeostasis in epicardial adipose tissue of heart failure Zou, Rongjun Zhang, Miao Zou, Zengxiao Shi, Wanting Tan, Songtao Wang, Chaojie Xu, Wenliu Jin, Jiaqi Milton, Stephen Chen, Yang Wang, Ge Fan, Xiaoping Int J Biol Sci Research Paper Epicardial adipose tissue (EAT) is a unique visceral fat reservoir that shares an immune microenvironment without a distinct boundary with myocardium. Increasingly, visceral fat has been studied as a secondary immune organ, and EAT is no exception in this regard. Cellular subsets of EAT are associated with disease development. In heart failure (HF) patients, however, the immune characteristics of EAT have rarely been studied, especially those non-immune cells related to the immune microenvironment. Herein, an analysis of seven EAT samples by single-cell RNA sequencing (scRNA-Seq) is presented here, including 1 neonate, 1 infant, 1 child, 2 adults with heart failure (Adults-HF) and 2 adult heart transplant donors as non-heart failure control (Adults-Non HF). Analysis of 51730 high-quality cells revealed eleven major cell types in EAT. For the first time, the pseudo-temporal reconstruction technique was employed to plot the cell trajectories of various major cell types (such as T lymphocytes, fibroblasts, endothelial cells, monocytes, and smooth muscle cells) in EAT across different developmental stages, achieving a single-cell resolution. The dynamic gene expression patterns of major cell types presented the immune characteristics of metabolism disorder of zinc and copper ions, and downregulated immune-related pathways in EAT of adult patients with HF. These data provide insights regarding HF immune dysregulation at the cellular level. Ivyspring International Publisher 2023-07-31 /pmc/articles/PMC10496493/ /pubmed/37705737 http://dx.doi.org/10.7150/ijbs.82844 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Zou, Rongjun
Zhang, Miao
Zou, Zengxiao
Shi, Wanting
Tan, Songtao
Wang, Chaojie
Xu, Wenliu
Jin, Jiaqi
Milton, Stephen
Chen, Yang
Wang, Ge
Fan, Xiaoping
Single-cell transcriptomics reveals zinc and copper ions homeostasis in epicardial adipose tissue of heart failure
title Single-cell transcriptomics reveals zinc and copper ions homeostasis in epicardial adipose tissue of heart failure
title_full Single-cell transcriptomics reveals zinc and copper ions homeostasis in epicardial adipose tissue of heart failure
title_fullStr Single-cell transcriptomics reveals zinc and copper ions homeostasis in epicardial adipose tissue of heart failure
title_full_unstemmed Single-cell transcriptomics reveals zinc and copper ions homeostasis in epicardial adipose tissue of heart failure
title_short Single-cell transcriptomics reveals zinc and copper ions homeostasis in epicardial adipose tissue of heart failure
title_sort single-cell transcriptomics reveals zinc and copper ions homeostasis in epicardial adipose tissue of heart failure
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10496493/
https://www.ncbi.nlm.nih.gov/pubmed/37705737
http://dx.doi.org/10.7150/ijbs.82844
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