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Simultaneous CAS9 editing of cpSRP43, LHCA6, and LHCA7 in Picochlorum celeri lowers chlorophyll levels and improves biomass productivity
High cellular pigment levels in dense microalgal cultures contribute to excess light absorption. To improve photosynthetic yields in the marine microalga Picochlorum celeri, CAS9 gene editing was used to target the molecular chaperone cpSRP43. Depigmented strains (>50% lower chlorophyll) were gen...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497401/ https://www.ncbi.nlm.nih.gov/pubmed/37711644 http://dx.doi.org/10.1002/pld3.530 |
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author | Krishnan, Anagha Cano, Melissa Karns, Devin A. Burch, Tyson A. Likhogrud, Maria Aqui, Moena Bailey, Shaun Verruto, John Lambert, William Kuzminov, Fedor Naghipor, Mahva Wang, Yingjun Ebmeier, Christopher C. Weissman, Joseph C. Posewitz, Matthew C. |
author_facet | Krishnan, Anagha Cano, Melissa Karns, Devin A. Burch, Tyson A. Likhogrud, Maria Aqui, Moena Bailey, Shaun Verruto, John Lambert, William Kuzminov, Fedor Naghipor, Mahva Wang, Yingjun Ebmeier, Christopher C. Weissman, Joseph C. Posewitz, Matthew C. |
author_sort | Krishnan, Anagha |
collection | PubMed |
description | High cellular pigment levels in dense microalgal cultures contribute to excess light absorption. To improve photosynthetic yields in the marine microalga Picochlorum celeri, CAS9 gene editing was used to target the molecular chaperone cpSRP43. Depigmented strains (>50% lower chlorophyll) were generated, with proteomics showing attenuated levels of most light harvesting complex (LHC) proteins. Gene editing generated two types of cpSRP43 transformants with distinct lower pigment phenotypes: (i) a transformant (Δsrp43) with both cpSRP43 diploid alleles modified to encode non‐functional polypeptides and (ii) a transformant (STR30309) with a 3 nt in‐frame insertion in one allele at the CAS9 cut site (non‐functional second allele), leading to expression of a modified cpSRP43. STR30309 has more chlorophyll than Δsrp43 but substantially less than wild type. To further decrease light absorption by photosystem I in STR30309, CAS9 editing was used to stack in disruptions of both LHCA6 and LHCA7 to generate STR30843, which has higher (5–24%) productivities relative to wild type in solar‐simulating bioreactors. Maximal productivities required frequent partial harvests throughout the day. For STR30843, exemplary diel bioreactor yields of ~50 g m(−2) day(−1) were attained. Our results demonstrate diel productivity gains in P. celeri by lowering pigment levels. |
format | Online Article Text |
id | pubmed-10497401 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104974012023-09-14 Simultaneous CAS9 editing of cpSRP43, LHCA6, and LHCA7 in Picochlorum celeri lowers chlorophyll levels and improves biomass productivity Krishnan, Anagha Cano, Melissa Karns, Devin A. Burch, Tyson A. Likhogrud, Maria Aqui, Moena Bailey, Shaun Verruto, John Lambert, William Kuzminov, Fedor Naghipor, Mahva Wang, Yingjun Ebmeier, Christopher C. Weissman, Joseph C. Posewitz, Matthew C. Plant Direct Research Articles High cellular pigment levels in dense microalgal cultures contribute to excess light absorption. To improve photosynthetic yields in the marine microalga Picochlorum celeri, CAS9 gene editing was used to target the molecular chaperone cpSRP43. Depigmented strains (>50% lower chlorophyll) were generated, with proteomics showing attenuated levels of most light harvesting complex (LHC) proteins. Gene editing generated two types of cpSRP43 transformants with distinct lower pigment phenotypes: (i) a transformant (Δsrp43) with both cpSRP43 diploid alleles modified to encode non‐functional polypeptides and (ii) a transformant (STR30309) with a 3 nt in‐frame insertion in one allele at the CAS9 cut site (non‐functional second allele), leading to expression of a modified cpSRP43. STR30309 has more chlorophyll than Δsrp43 but substantially less than wild type. To further decrease light absorption by photosystem I in STR30309, CAS9 editing was used to stack in disruptions of both LHCA6 and LHCA7 to generate STR30843, which has higher (5–24%) productivities relative to wild type in solar‐simulating bioreactors. Maximal productivities required frequent partial harvests throughout the day. For STR30843, exemplary diel bioreactor yields of ~50 g m(−2) day(−1) were attained. Our results demonstrate diel productivity gains in P. celeri by lowering pigment levels. John Wiley and Sons Inc. 2023-09-12 /pmc/articles/PMC10497401/ /pubmed/37711644 http://dx.doi.org/10.1002/pld3.530 Text en © 2023 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Krishnan, Anagha Cano, Melissa Karns, Devin A. Burch, Tyson A. Likhogrud, Maria Aqui, Moena Bailey, Shaun Verruto, John Lambert, William Kuzminov, Fedor Naghipor, Mahva Wang, Yingjun Ebmeier, Christopher C. Weissman, Joseph C. Posewitz, Matthew C. Simultaneous CAS9 editing of cpSRP43, LHCA6, and LHCA7 in Picochlorum celeri lowers chlorophyll levels and improves biomass productivity |
title | Simultaneous CAS9 editing of cpSRP43, LHCA6, and LHCA7 in Picochlorum celeri lowers chlorophyll levels and improves biomass productivity |
title_full | Simultaneous CAS9 editing of cpSRP43, LHCA6, and LHCA7 in Picochlorum celeri lowers chlorophyll levels and improves biomass productivity |
title_fullStr | Simultaneous CAS9 editing of cpSRP43, LHCA6, and LHCA7 in Picochlorum celeri lowers chlorophyll levels and improves biomass productivity |
title_full_unstemmed | Simultaneous CAS9 editing of cpSRP43, LHCA6, and LHCA7 in Picochlorum celeri lowers chlorophyll levels and improves biomass productivity |
title_short | Simultaneous CAS9 editing of cpSRP43, LHCA6, and LHCA7 in Picochlorum celeri lowers chlorophyll levels and improves biomass productivity |
title_sort | simultaneous cas9 editing of cpsrp43, lhca6, and lhca7 in picochlorum celeri lowers chlorophyll levels and improves biomass productivity |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497401/ https://www.ncbi.nlm.nih.gov/pubmed/37711644 http://dx.doi.org/10.1002/pld3.530 |
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