An easy adjustment of instrument settings (‘Peak MALDI’) improves identification of organisms by MALDI-ToF mass spectrometry

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) is a mature technology with ‘auto-execute’ instrument settings and peak processing parameters tailored for rapid bacterial identification. Adoption for other organisms has been problematic, with optimisation...

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Autores principales: Nellessen, Christopher M., Nehl, David B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497524/
https://www.ncbi.nlm.nih.gov/pubmed/37700004
http://dx.doi.org/10.1038/s41598-023-42328-2
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author Nellessen, Christopher M.
Nehl, David B.
author_facet Nellessen, Christopher M.
Nehl, David B.
author_sort Nellessen, Christopher M.
collection PubMed
description Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) is a mature technology with ‘auto-execute’ instrument settings and peak processing parameters tailored for rapid bacterial identification. Adoption for other organisms has been problematic, with optimisation efforts focusing on sample preparation. Using the Bruker MALDI Biotyper, we demonstrate ‘Peak MALDI’: easily-applied settings that immediately enhance sensitivity, improve spectrum quality, and increase identification confidence for any target, establishing its potential value for all MALDI-ToF MS systems.
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spelling pubmed-104975242023-09-14 An easy adjustment of instrument settings (‘Peak MALDI’) improves identification of organisms by MALDI-ToF mass spectrometry Nellessen, Christopher M. Nehl, David B. Sci Rep Article Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) is a mature technology with ‘auto-execute’ instrument settings and peak processing parameters tailored for rapid bacterial identification. Adoption for other organisms has been problematic, with optimisation efforts focusing on sample preparation. Using the Bruker MALDI Biotyper, we demonstrate ‘Peak MALDI’: easily-applied settings that immediately enhance sensitivity, improve spectrum quality, and increase identification confidence for any target, establishing its potential value for all MALDI-ToF MS systems. Nature Publishing Group UK 2023-09-12 /pmc/articles/PMC10497524/ /pubmed/37700004 http://dx.doi.org/10.1038/s41598-023-42328-2 Text en © Crown 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Nellessen, Christopher M.
Nehl, David B.
An easy adjustment of instrument settings (‘Peak MALDI’) improves identification of organisms by MALDI-ToF mass spectrometry
title An easy adjustment of instrument settings (‘Peak MALDI’) improves identification of organisms by MALDI-ToF mass spectrometry
title_full An easy adjustment of instrument settings (‘Peak MALDI’) improves identification of organisms by MALDI-ToF mass spectrometry
title_fullStr An easy adjustment of instrument settings (‘Peak MALDI’) improves identification of organisms by MALDI-ToF mass spectrometry
title_full_unstemmed An easy adjustment of instrument settings (‘Peak MALDI’) improves identification of organisms by MALDI-ToF mass spectrometry
title_short An easy adjustment of instrument settings (‘Peak MALDI’) improves identification of organisms by MALDI-ToF mass spectrometry
title_sort easy adjustment of instrument settings (‘peak maldi’) improves identification of organisms by maldi-tof mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497524/
https://www.ncbi.nlm.nih.gov/pubmed/37700004
http://dx.doi.org/10.1038/s41598-023-42328-2
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