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Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells

Human embryonic stem cells (hESCs) have unique abilities that enable their use in cell therapy, disease modeling, and drug development. Their derivation is usually performed using a feeder layer, which is undefined and can potentially cause a contamination by xeno components, therefore there is a te...

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Autores principales: Souralova, Tereza, Hulinova, Daniela, Jeseta, Michal, Ventruba, Pavel, Hampl, Ales, Koutna, Irena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497536/
https://www.ncbi.nlm.nih.gov/pubmed/37700192
http://dx.doi.org/10.1038/s41598-023-42236-5
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author Souralova, Tereza
Hulinova, Daniela
Jeseta, Michal
Ventruba, Pavel
Hampl, Ales
Koutna, Irena
author_facet Souralova, Tereza
Hulinova, Daniela
Jeseta, Michal
Ventruba, Pavel
Hampl, Ales
Koutna, Irena
author_sort Souralova, Tereza
collection PubMed
description Human embryonic stem cells (hESCs) have unique abilities that enable their use in cell therapy, disease modeling, and drug development. Their derivation is usually performed using a feeder layer, which is undefined and can potentially cause a contamination by xeno components, therefore there is a tendency to replace feeders with xeno-free defined substrates in recent years. Three hESC lines were successfully derived on the vitronectin with a truncated N-terminus (VTN-N) in combination with E-cadherin in xeno-free conditions for the first time, and their undifferentiated state, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers were confirmed. These results support the conclusion that the VTN-N/E-cadherin is a suitable substrate for the xeno-free derivation of hESCs and can be used for the derivation of hESCs according to good manufacturing practices.
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spelling pubmed-104975362023-09-14 Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells Souralova, Tereza Hulinova, Daniela Jeseta, Michal Ventruba, Pavel Hampl, Ales Koutna, Irena Sci Rep Article Human embryonic stem cells (hESCs) have unique abilities that enable their use in cell therapy, disease modeling, and drug development. Their derivation is usually performed using a feeder layer, which is undefined and can potentially cause a contamination by xeno components, therefore there is a tendency to replace feeders with xeno-free defined substrates in recent years. Three hESC lines were successfully derived on the vitronectin with a truncated N-terminus (VTN-N) in combination with E-cadherin in xeno-free conditions for the first time, and their undifferentiated state, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers were confirmed. These results support the conclusion that the VTN-N/E-cadherin is a suitable substrate for the xeno-free derivation of hESCs and can be used for the derivation of hESCs according to good manufacturing practices. Nature Publishing Group UK 2023-09-12 /pmc/articles/PMC10497536/ /pubmed/37700192 http://dx.doi.org/10.1038/s41598-023-42236-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Souralova, Tereza
Hulinova, Daniela
Jeseta, Michal
Ventruba, Pavel
Hampl, Ales
Koutna, Irena
Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells
title Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells
title_full Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells
title_fullStr Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells
title_full_unstemmed Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells
title_short Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells
title_sort truncated vitronectin with e-cadherin enables the xeno-free derivation of human embryonic stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497536/
https://www.ncbi.nlm.nih.gov/pubmed/37700192
http://dx.doi.org/10.1038/s41598-023-42236-5
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