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A novel method to quantify perivascular space enlargement near the syrinx in a rodent model of post-traumatic syringomyelia

Posttraumatic syringomyelia (PTS) is an enigmatic condition characterized by the development of fluid-filled cysts (syrinxes) within the spinal cord. Perivascular spaces (PVS) are a critical component of fluid transport within the central nervous system (CNS), with dilated PVSs variably implicated i...

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Autores principales: Johnson, Liam, Bartlett-Tomasetig, Florence, Fok, Sandra, Whan, Renee, Berliner, Joel, Hemley, Sarah J., Stoodley, Marcus A., Bilston, Lynne E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497578/
https://www.ncbi.nlm.nih.gov/pubmed/37700036
http://dx.doi.org/10.1038/s41598-023-42275-y
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author Johnson, Liam
Bartlett-Tomasetig, Florence
Fok, Sandra
Whan, Renee
Berliner, Joel
Hemley, Sarah J.
Stoodley, Marcus A.
Bilston, Lynne E.
author_facet Johnson, Liam
Bartlett-Tomasetig, Florence
Fok, Sandra
Whan, Renee
Berliner, Joel
Hemley, Sarah J.
Stoodley, Marcus A.
Bilston, Lynne E.
author_sort Johnson, Liam
collection PubMed
description Posttraumatic syringomyelia (PTS) is an enigmatic condition characterized by the development of fluid-filled cysts (syrinxes) within the spinal cord. Perivascular spaces (PVS) are a critical component of fluid transport within the central nervous system (CNS), with dilated PVSs variably implicated in the pathogenesis of syringomyelia. The extent and spatial distribution of dilated PVSs in syringomyelia, however, remains unclear. This study aims to develop a method to assess PVS dimensions across multiple spinal cord segments in rats with PTS. Syrinxes were induced in two Sprague–Dawley rats at C6/7 with computer-controlled motorized spinal cord impaction; two control rats underwent sham laminectomies. Spinal cord segments were obtained at C4, C6 and C8, cleared via tissue clearing protocols, stained with immunofluorescent antibodies and imaged under confocal microscopy. Qualitative and quantitative analyses of PVS size were performed. Arteriolar PVSs were enlarged in the perisyringeal region of the spinal cord, compared to the control cord. No PVS enlargement was observed above or below the syrinx. These results confirm previous incidental findings of enlarged PVSs in the perisyringeal region, providing new insights into PVS dimensions across multiple spinal segments, and providing a novel method for quantifying spinal cord perivascular space size distributions.
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spelling pubmed-104975782023-09-14 A novel method to quantify perivascular space enlargement near the syrinx in a rodent model of post-traumatic syringomyelia Johnson, Liam Bartlett-Tomasetig, Florence Fok, Sandra Whan, Renee Berliner, Joel Hemley, Sarah J. Stoodley, Marcus A. Bilston, Lynne E. Sci Rep Article Posttraumatic syringomyelia (PTS) is an enigmatic condition characterized by the development of fluid-filled cysts (syrinxes) within the spinal cord. Perivascular spaces (PVS) are a critical component of fluid transport within the central nervous system (CNS), with dilated PVSs variably implicated in the pathogenesis of syringomyelia. The extent and spatial distribution of dilated PVSs in syringomyelia, however, remains unclear. This study aims to develop a method to assess PVS dimensions across multiple spinal cord segments in rats with PTS. Syrinxes were induced in two Sprague–Dawley rats at C6/7 with computer-controlled motorized spinal cord impaction; two control rats underwent sham laminectomies. Spinal cord segments were obtained at C4, C6 and C8, cleared via tissue clearing protocols, stained with immunofluorescent antibodies and imaged under confocal microscopy. Qualitative and quantitative analyses of PVS size were performed. Arteriolar PVSs were enlarged in the perisyringeal region of the spinal cord, compared to the control cord. No PVS enlargement was observed above or below the syrinx. These results confirm previous incidental findings of enlarged PVSs in the perisyringeal region, providing new insights into PVS dimensions across multiple spinal segments, and providing a novel method for quantifying spinal cord perivascular space size distributions. Nature Publishing Group UK 2023-09-12 /pmc/articles/PMC10497578/ /pubmed/37700036 http://dx.doi.org/10.1038/s41598-023-42275-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Johnson, Liam
Bartlett-Tomasetig, Florence
Fok, Sandra
Whan, Renee
Berliner, Joel
Hemley, Sarah J.
Stoodley, Marcus A.
Bilston, Lynne E.
A novel method to quantify perivascular space enlargement near the syrinx in a rodent model of post-traumatic syringomyelia
title A novel method to quantify perivascular space enlargement near the syrinx in a rodent model of post-traumatic syringomyelia
title_full A novel method to quantify perivascular space enlargement near the syrinx in a rodent model of post-traumatic syringomyelia
title_fullStr A novel method to quantify perivascular space enlargement near the syrinx in a rodent model of post-traumatic syringomyelia
title_full_unstemmed A novel method to quantify perivascular space enlargement near the syrinx in a rodent model of post-traumatic syringomyelia
title_short A novel method to quantify perivascular space enlargement near the syrinx in a rodent model of post-traumatic syringomyelia
title_sort novel method to quantify perivascular space enlargement near the syrinx in a rodent model of post-traumatic syringomyelia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497578/
https://www.ncbi.nlm.nih.gov/pubmed/37700036
http://dx.doi.org/10.1038/s41598-023-42275-y
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