Cargando…
PacBio next-generation sequencing uncovers Apicomplexa diversity in different habitats
The phylum Apicomplexa comprises a large group of intracellular protozoan parasites. These microorganisms are known to infect a variety of vertebrate and invertebrate hosts, leading to significant medical and veterinary conditions such as toxoplasmosis, cryptosporidiosis, theileriosis, and eimeriosi...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497610/ https://www.ncbi.nlm.nih.gov/pubmed/37699953 http://dx.doi.org/10.1038/s41598-023-40895-y |
_version_ | 1785105342101716992 |
---|---|
author | Gad, Mahmoud Fawzy, Mariam E. Al-Herrawy, Ahmad Z. Abdo, Sayeda M. Nabet, Noura Hu, Anyi |
author_facet | Gad, Mahmoud Fawzy, Mariam E. Al-Herrawy, Ahmad Z. Abdo, Sayeda M. Nabet, Noura Hu, Anyi |
author_sort | Gad, Mahmoud |
collection | PubMed |
description | The phylum Apicomplexa comprises a large group of intracellular protozoan parasites. These microorganisms are known to infect a variety of vertebrate and invertebrate hosts, leading to significant medical and veterinary conditions such as toxoplasmosis, cryptosporidiosis, theileriosis, and eimeriosis. Despite their importance, comprehensive data on their diversity and distribution, especially in riverine environments, remain scant. To bridge this knowledge gap, we utilized next-generation high-throughput 18S rRNA amplicon sequencing powered by PacBio technology to explore the diversity and composition of the Apicomplexa taxa. Principal component analysis (PCA) and principal coordinate analysis (PCoA) indicated the habitat heterogeneity for the physicochemical parameters and the Apicomplexa community. These results were supported by PERMANOVA (P < 0.001), ANOSIM (P < 0.001), Cluster analysis, and Venn diagram. Dominant genera of Apicomplexa in inlet samples included Gregarina (38.54%), Cryptosporidium (32.29%), and Leidyana (11.90%). In contrast, outlet samples were dominated by Babesia, Cryptosporidium, and Theileria. While surface water samples revealed 16% and 8.33% relative abundance of Toxoplasma and Cryptosporidium, respectively. To our knowledge, the next-generation high throughput sequencing covered a wide range of parasites in Egypt for the first time, which could be useful for legislation of the standards for drinking water and wastewater reuse. |
format | Online Article Text |
id | pubmed-10497610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-104976102023-09-14 PacBio next-generation sequencing uncovers Apicomplexa diversity in different habitats Gad, Mahmoud Fawzy, Mariam E. Al-Herrawy, Ahmad Z. Abdo, Sayeda M. Nabet, Noura Hu, Anyi Sci Rep Article The phylum Apicomplexa comprises a large group of intracellular protozoan parasites. These microorganisms are known to infect a variety of vertebrate and invertebrate hosts, leading to significant medical and veterinary conditions such as toxoplasmosis, cryptosporidiosis, theileriosis, and eimeriosis. Despite their importance, comprehensive data on their diversity and distribution, especially in riverine environments, remain scant. To bridge this knowledge gap, we utilized next-generation high-throughput 18S rRNA amplicon sequencing powered by PacBio technology to explore the diversity and composition of the Apicomplexa taxa. Principal component analysis (PCA) and principal coordinate analysis (PCoA) indicated the habitat heterogeneity for the physicochemical parameters and the Apicomplexa community. These results were supported by PERMANOVA (P < 0.001), ANOSIM (P < 0.001), Cluster analysis, and Venn diagram. Dominant genera of Apicomplexa in inlet samples included Gregarina (38.54%), Cryptosporidium (32.29%), and Leidyana (11.90%). In contrast, outlet samples were dominated by Babesia, Cryptosporidium, and Theileria. While surface water samples revealed 16% and 8.33% relative abundance of Toxoplasma and Cryptosporidium, respectively. To our knowledge, the next-generation high throughput sequencing covered a wide range of parasites in Egypt for the first time, which could be useful for legislation of the standards for drinking water and wastewater reuse. Nature Publishing Group UK 2023-09-12 /pmc/articles/PMC10497610/ /pubmed/37699953 http://dx.doi.org/10.1038/s41598-023-40895-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Gad, Mahmoud Fawzy, Mariam E. Al-Herrawy, Ahmad Z. Abdo, Sayeda M. Nabet, Noura Hu, Anyi PacBio next-generation sequencing uncovers Apicomplexa diversity in different habitats |
title | PacBio next-generation sequencing uncovers Apicomplexa diversity in different habitats |
title_full | PacBio next-generation sequencing uncovers Apicomplexa diversity in different habitats |
title_fullStr | PacBio next-generation sequencing uncovers Apicomplexa diversity in different habitats |
title_full_unstemmed | PacBio next-generation sequencing uncovers Apicomplexa diversity in different habitats |
title_short | PacBio next-generation sequencing uncovers Apicomplexa diversity in different habitats |
title_sort | pacbio next-generation sequencing uncovers apicomplexa diversity in different habitats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497610/ https://www.ncbi.nlm.nih.gov/pubmed/37699953 http://dx.doi.org/10.1038/s41598-023-40895-y |
work_keys_str_mv | AT gadmahmoud pacbionextgenerationsequencinguncoversapicomplexadiversityindifferenthabitats AT fawzymariame pacbionextgenerationsequencinguncoversapicomplexadiversityindifferenthabitats AT alherrawyahmadz pacbionextgenerationsequencinguncoversapicomplexadiversityindifferenthabitats AT abdosayedam pacbionextgenerationsequencinguncoversapicomplexadiversityindifferenthabitats AT nabetnoura pacbionextgenerationsequencinguncoversapicomplexadiversityindifferenthabitats AT huanyi pacbionextgenerationsequencinguncoversapicomplexadiversityindifferenthabitats |