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A novel method for irrigating plants, tracking water use, and imposing water deficits in controlled environments
The study of genomic control of drought tolerance in crops requires techniques to impose well defined and consistent levels of drought stress and efficiently measure single-plant water use for hundreds of experimental units over timescales of several months. Traditional gravimetric methods are extre...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497755/ https://www.ncbi.nlm.nih.gov/pubmed/37711304 http://dx.doi.org/10.3389/fpls.2023.1201102 |
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author | Cichello, Alex Bruch, Austin Earl, Hugh J. |
author_facet | Cichello, Alex Bruch, Austin Earl, Hugh J. |
author_sort | Cichello, Alex |
collection | PubMed |
description | The study of genomic control of drought tolerance in crops requires techniques to impose well defined and consistent levels of drought stress and efficiently measure single-plant water use for hundreds of experimental units over timescales of several months. Traditional gravimetric methods are extremely labor intensive or require expensive technology, and are subject to other errors. This study demonstrates a low-cost, passive, bottom-watered system that is easily scaled for high-throughput phenotyping. The soil water content in the pots is controlled by altering the water table height in an underlying wicking bed via a float valve. The resulting soil moisture profile is then maintained passively as water withdrawn by the plant is replaced by upward movement of water from the wicking bed, which is fed from a reservoir via the float valve. The single-plant water use can be directly measured over time intervals from one to several days by observing the water level in the reservoir. Using this method, four different drought stress levels were induced in pots containing soybean (Glycine max (L.) Merr.), producing four statistically distinct groups for shoot dry weight and seed yield, as well as clear treatment effects for other relevant parameters, including root:shoot dry weight ratio, pod number, cumulative water use, and water use efficiency. This system has a broad range of applications, and should increase feasibility of high-throughput phenotyping efforts for plant drought tolerance traits. |
format | Online Article Text |
id | pubmed-10497755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104977552023-09-14 A novel method for irrigating plants, tracking water use, and imposing water deficits in controlled environments Cichello, Alex Bruch, Austin Earl, Hugh J. Front Plant Sci Plant Science The study of genomic control of drought tolerance in crops requires techniques to impose well defined and consistent levels of drought stress and efficiently measure single-plant water use for hundreds of experimental units over timescales of several months. Traditional gravimetric methods are extremely labor intensive or require expensive technology, and are subject to other errors. This study demonstrates a low-cost, passive, bottom-watered system that is easily scaled for high-throughput phenotyping. The soil water content in the pots is controlled by altering the water table height in an underlying wicking bed via a float valve. The resulting soil moisture profile is then maintained passively as water withdrawn by the plant is replaced by upward movement of water from the wicking bed, which is fed from a reservoir via the float valve. The single-plant water use can be directly measured over time intervals from one to several days by observing the water level in the reservoir. Using this method, four different drought stress levels were induced in pots containing soybean (Glycine max (L.) Merr.), producing four statistically distinct groups for shoot dry weight and seed yield, as well as clear treatment effects for other relevant parameters, including root:shoot dry weight ratio, pod number, cumulative water use, and water use efficiency. This system has a broad range of applications, and should increase feasibility of high-throughput phenotyping efforts for plant drought tolerance traits. Frontiers Media S.A. 2023-08-29 /pmc/articles/PMC10497755/ /pubmed/37711304 http://dx.doi.org/10.3389/fpls.2023.1201102 Text en Copyright © 2023 Cichello, Bruch and Earl https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Cichello, Alex Bruch, Austin Earl, Hugh J. A novel method for irrigating plants, tracking water use, and imposing water deficits in controlled environments |
title | A novel method for irrigating plants, tracking water use, and imposing water deficits in controlled environments |
title_full | A novel method for irrigating plants, tracking water use, and imposing water deficits in controlled environments |
title_fullStr | A novel method for irrigating plants, tracking water use, and imposing water deficits in controlled environments |
title_full_unstemmed | A novel method for irrigating plants, tracking water use, and imposing water deficits in controlled environments |
title_short | A novel method for irrigating plants, tracking water use, and imposing water deficits in controlled environments |
title_sort | novel method for irrigating plants, tracking water use, and imposing water deficits in controlled environments |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497755/ https://www.ncbi.nlm.nih.gov/pubmed/37711304 http://dx.doi.org/10.3389/fpls.2023.1201102 |
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