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Comparative genomic and transcriptomic analyses of African swine fever virus strains
African swine fever (ASF) is the most devastating disease caused by the African swine fever virus (ASFV), impacting the pig industry worldwide and threatening food security and biodiversity. Although two vaccines have been approved in Vietnam to combat ASFV, the complexity of the virus, with its num...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Research Network of Computational and Structural Biotechnology
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497913/ https://www.ncbi.nlm.nih.gov/pubmed/37711186 http://dx.doi.org/10.1016/j.csbj.2023.08.028 |
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author | Lu, Peng Zhou, Jiaqiao Wei, Sibo Takada, Konosuke Masutani, Hayato Okuda, Suguru Okamoto, Ken Suzuki, Michio Kitamura, Tomoya Masujin, Kentaro Kokuho, Takehiro Itoh, Hideaki Nagata, Koji |
author_facet | Lu, Peng Zhou, Jiaqiao Wei, Sibo Takada, Konosuke Masutani, Hayato Okuda, Suguru Okamoto, Ken Suzuki, Michio Kitamura, Tomoya Masujin, Kentaro Kokuho, Takehiro Itoh, Hideaki Nagata, Koji |
author_sort | Lu, Peng |
collection | PubMed |
description | African swine fever (ASF) is the most devastating disease caused by the African swine fever virus (ASFV), impacting the pig industry worldwide and threatening food security and biodiversity. Although two vaccines have been approved in Vietnam to combat ASFV, the complexity of the virus, with its numerous open reading frames (ORFs), necessitates a more diverse vaccine strategy. Therefore, we focused on identifying and investigating the potential vaccine targets for developing a broad-spectrum defense against the virus. This study collected the genomic and/or transcriptomic data of different ASFV strains, specifically from in vitro studies, focusing on comparisons between genotypes I, II, and X, from the National Center for Biotechnology Information (NCBI) database. The comprehensive analysis of the genomic and transcriptomic differences between high- and low-virulence strains revealed six early genes, 13 late genes, and six short genes as potentially essential ORFs associated with high-virulence. In addition, many other ORFs (e.g., 14 multigene family members) are worth investigating. The results of this study provided candidate ORFs for developing ASF vaccines and therapies. |
format | Online Article Text |
id | pubmed-10497913 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Research Network of Computational and Structural Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-104979132023-09-14 Comparative genomic and transcriptomic analyses of African swine fever virus strains Lu, Peng Zhou, Jiaqiao Wei, Sibo Takada, Konosuke Masutani, Hayato Okuda, Suguru Okamoto, Ken Suzuki, Michio Kitamura, Tomoya Masujin, Kentaro Kokuho, Takehiro Itoh, Hideaki Nagata, Koji Comput Struct Biotechnol J Research Article African swine fever (ASF) is the most devastating disease caused by the African swine fever virus (ASFV), impacting the pig industry worldwide and threatening food security and biodiversity. Although two vaccines have been approved in Vietnam to combat ASFV, the complexity of the virus, with its numerous open reading frames (ORFs), necessitates a more diverse vaccine strategy. Therefore, we focused on identifying and investigating the potential vaccine targets for developing a broad-spectrum defense against the virus. This study collected the genomic and/or transcriptomic data of different ASFV strains, specifically from in vitro studies, focusing on comparisons between genotypes I, II, and X, from the National Center for Biotechnology Information (NCBI) database. The comprehensive analysis of the genomic and transcriptomic differences between high- and low-virulence strains revealed six early genes, 13 late genes, and six short genes as potentially essential ORFs associated with high-virulence. In addition, many other ORFs (e.g., 14 multigene family members) are worth investigating. The results of this study provided candidate ORFs for developing ASF vaccines and therapies. Research Network of Computational and Structural Biotechnology 2023-08-29 /pmc/articles/PMC10497913/ /pubmed/37711186 http://dx.doi.org/10.1016/j.csbj.2023.08.028 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Lu, Peng Zhou, Jiaqiao Wei, Sibo Takada, Konosuke Masutani, Hayato Okuda, Suguru Okamoto, Ken Suzuki, Michio Kitamura, Tomoya Masujin, Kentaro Kokuho, Takehiro Itoh, Hideaki Nagata, Koji Comparative genomic and transcriptomic analyses of African swine fever virus strains |
title | Comparative genomic and transcriptomic analyses of African swine fever virus strains |
title_full | Comparative genomic and transcriptomic analyses of African swine fever virus strains |
title_fullStr | Comparative genomic and transcriptomic analyses of African swine fever virus strains |
title_full_unstemmed | Comparative genomic and transcriptomic analyses of African swine fever virus strains |
title_short | Comparative genomic and transcriptomic analyses of African swine fever virus strains |
title_sort | comparative genomic and transcriptomic analyses of african swine fever virus strains |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497913/ https://www.ncbi.nlm.nih.gov/pubmed/37711186 http://dx.doi.org/10.1016/j.csbj.2023.08.028 |
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