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The first ITS2 sequence data set of eDNA from honey of Malaysian giant honeybees (Apis dorsata) and stingless bees (Heterotrigona itama) reveals plant species diversity

OBJECTIVES: Pollen is a useful tool for identifying the provenance and complex ecosystems surrounding honey production in Malaysian forests. As native key pollinators in Malaysia, Apis dorsata and Heterotrigona itama forage on various plant/pollen species to collect honey. This study aims to generat...

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Autores principales: Huda, Nurul, ullah, Saeed, Wahab, Roswanira Abdul, Lani, Mohd Nizam, Daud, Nur Hardy Abu, Shariff, Amir Husni Mohd, Ismail, Norjihada Izzah, Hamid, Azzmer Azzar Abdul, Mohamad, Mohd Azrul Naim, Huyop, Fahrul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10498584/
https://www.ncbi.nlm.nih.gov/pubmed/37700361
http://dx.doi.org/10.1186/s13104-023-06495-9
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author Huda, Nurul
ullah, Saeed
Wahab, Roswanira Abdul
Lani, Mohd Nizam
Daud, Nur Hardy Abu
Shariff, Amir Husni Mohd
Ismail, Norjihada Izzah
Hamid, Azzmer Azzar Abdul
Mohamad, Mohd Azrul Naim
Huyop, Fahrul
author_facet Huda, Nurul
ullah, Saeed
Wahab, Roswanira Abdul
Lani, Mohd Nizam
Daud, Nur Hardy Abu
Shariff, Amir Husni Mohd
Ismail, Norjihada Izzah
Hamid, Azzmer Azzar Abdul
Mohamad, Mohd Azrul Naim
Huyop, Fahrul
author_sort Huda, Nurul
collection PubMed
description OBJECTIVES: Pollen is a useful tool for identifying the provenance and complex ecosystems surrounding honey production in Malaysian forests. As native key pollinators in Malaysia, Apis dorsata and Heterotrigona itama forage on various plant/pollen species to collect honey. This study aims to generate a dataset that uncovers the presence of these plant/pollen species and their relative abundance in the honey of A. dorsata and H. itama. The information gathered from this study can be used to determine the geographical and botanical origin and authenticity of the honey produced by these two species. RESULTS: Sequence data were obtained for both A. dorsata and H. itama. The raw sequence data for A. dorsata was 5 Mb, which was assembled into 5 contigs with a size of 6,098,728 bp, an N50 of 15,534, and a GC average of 57.42. Similarly, the raw sequence data for H. itama was 6.3 Mb, which was assembled into 11 contigs with a size of 7,642,048 bp, an N50 of 17,180, and a GC average of 55.38. In the honey sample of A. dorsata, we identified five different plant/pollen species, with only one of the five species exhibiting a relative abundance of less than 1%. For H. itama, we identified seven different plant/pollen species, with only three of the species exhibiting a relative abundance of less than 1%. All of the identified plant species were native to Peninsular Malaysia, especially the East Coast area of Terengganu. DATA DESCRIPTION: Our data offers valuable insights into honey’s geographical and botanical origin and authenticity. Metagenomic studies could help identify the plant species that honeybees forage and provide preliminary data for researchers studying the biological development of A. dorsata and H. itama. The identification of various flowers from the eDNA of honey that are known for their medicinal properties could aid in regional honey with accurate product origin labeling, which is crucial for guaranteeing product authenticity to consumers.
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spelling pubmed-104985842023-09-14 The first ITS2 sequence data set of eDNA from honey of Malaysian giant honeybees (Apis dorsata) and stingless bees (Heterotrigona itama) reveals plant species diversity Huda, Nurul ullah, Saeed Wahab, Roswanira Abdul Lani, Mohd Nizam Daud, Nur Hardy Abu Shariff, Amir Husni Mohd Ismail, Norjihada Izzah Hamid, Azzmer Azzar Abdul Mohamad, Mohd Azrul Naim Huyop, Fahrul BMC Res Notes Research Note OBJECTIVES: Pollen is a useful tool for identifying the provenance and complex ecosystems surrounding honey production in Malaysian forests. As native key pollinators in Malaysia, Apis dorsata and Heterotrigona itama forage on various plant/pollen species to collect honey. This study aims to generate a dataset that uncovers the presence of these plant/pollen species and their relative abundance in the honey of A. dorsata and H. itama. The information gathered from this study can be used to determine the geographical and botanical origin and authenticity of the honey produced by these two species. RESULTS: Sequence data were obtained for both A. dorsata and H. itama. The raw sequence data for A. dorsata was 5 Mb, which was assembled into 5 contigs with a size of 6,098,728 bp, an N50 of 15,534, and a GC average of 57.42. Similarly, the raw sequence data for H. itama was 6.3 Mb, which was assembled into 11 contigs with a size of 7,642,048 bp, an N50 of 17,180, and a GC average of 55.38. In the honey sample of A. dorsata, we identified five different plant/pollen species, with only one of the five species exhibiting a relative abundance of less than 1%. For H. itama, we identified seven different plant/pollen species, with only three of the species exhibiting a relative abundance of less than 1%. All of the identified plant species were native to Peninsular Malaysia, especially the East Coast area of Terengganu. DATA DESCRIPTION: Our data offers valuable insights into honey’s geographical and botanical origin and authenticity. Metagenomic studies could help identify the plant species that honeybees forage and provide preliminary data for researchers studying the biological development of A. dorsata and H. itama. The identification of various flowers from the eDNA of honey that are known for their medicinal properties could aid in regional honey with accurate product origin labeling, which is crucial for guaranteeing product authenticity to consumers. BioMed Central 2023-09-12 /pmc/articles/PMC10498584/ /pubmed/37700361 http://dx.doi.org/10.1186/s13104-023-06495-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Huda, Nurul
ullah, Saeed
Wahab, Roswanira Abdul
Lani, Mohd Nizam
Daud, Nur Hardy Abu
Shariff, Amir Husni Mohd
Ismail, Norjihada Izzah
Hamid, Azzmer Azzar Abdul
Mohamad, Mohd Azrul Naim
Huyop, Fahrul
The first ITS2 sequence data set of eDNA from honey of Malaysian giant honeybees (Apis dorsata) and stingless bees (Heterotrigona itama) reveals plant species diversity
title The first ITS2 sequence data set of eDNA from honey of Malaysian giant honeybees (Apis dorsata) and stingless bees (Heterotrigona itama) reveals plant species diversity
title_full The first ITS2 sequence data set of eDNA from honey of Malaysian giant honeybees (Apis dorsata) and stingless bees (Heterotrigona itama) reveals plant species diversity
title_fullStr The first ITS2 sequence data set of eDNA from honey of Malaysian giant honeybees (Apis dorsata) and stingless bees (Heterotrigona itama) reveals plant species diversity
title_full_unstemmed The first ITS2 sequence data set of eDNA from honey of Malaysian giant honeybees (Apis dorsata) and stingless bees (Heterotrigona itama) reveals plant species diversity
title_short The first ITS2 sequence data set of eDNA from honey of Malaysian giant honeybees (Apis dorsata) and stingless bees (Heterotrigona itama) reveals plant species diversity
title_sort first its2 sequence data set of edna from honey of malaysian giant honeybees (apis dorsata) and stingless bees (heterotrigona itama) reveals plant species diversity
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10498584/
https://www.ncbi.nlm.nih.gov/pubmed/37700361
http://dx.doi.org/10.1186/s13104-023-06495-9
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