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Triplet states in the reaction center of Photosystem II

In oxygenic photosynthesis sunlight is harvested and funneled as excitation energy into the reaction center (RC) of Photosystem II (PSII), the site of primary charge separation that initiates the photosynthetic electron transfer chain. The chlorophyll Chl(D1) pigment of the RC is the primary electro...

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Detalles Bibliográficos
Autores principales: Bhattacharjee, Sinjini, Neese, Frank, Pantazis, Dimitrios A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10498673/
https://www.ncbi.nlm.nih.gov/pubmed/37712047
http://dx.doi.org/10.1039/d3sc02985a
Descripción
Sumario:In oxygenic photosynthesis sunlight is harvested and funneled as excitation energy into the reaction center (RC) of Photosystem II (PSII), the site of primary charge separation that initiates the photosynthetic electron transfer chain. The chlorophyll Chl(D1) pigment of the RC is the primary electron donor, forming a charge-separated radical pair with the vicinal pheophytin Pheo(D1) (Chl(D1)(+)Pheo(D1)(−)). To avert charge recombination, the electron is further transferred to plastoquinone Q(A), whereas the hole relaxes to a central pair of chlorophylls (P(D1)P(D2)), subsequently driving water oxidation. Spin-triplet states can form within the RC when forward electron transfer is inhibited or back reactions are favored. This can lead to formation of singlet dioxygen, with potential deleterious effects. Here we investigate the nature and properties of triplet states within the PSII RC using a multiscale quantum-mechanics/molecular-mechanics (QM/MM) approach. The low-energy spectrum of excited singlet and triplet states, of both local and charge-transfer nature, is compared using range-separated time-dependent density functional theory (TD-DFT). We further compute electron paramagnetic resonance properties (zero-field splitting parameters and hyperfine coupling constants) of relaxed triplet states and compare them with available experimental data. Moreover, the electrostatic modulation of excited state energetics and redox properties of RC pigments by the semiquinone Q(A)(−) is described. The results provide a detailed electronic-level understanding of triplet states within the PSII RC and form a refined basis for discussing primary and secondary electron transfer, charge recombination pathways, and possible photoprotection mechanisms in PSII.