Cargando…

Spatiotemporal diversity and community structure of cyanobacteria and associated bacteria in the large shallow subtropical Lake Okeechobee (Florida, United States)

Lake Okeechobee is a large eutrophic, shallow, subtropical lake in south Florida, United States. Due to decades of nutrient loading and phosphorus rich sediments, the lake is eutrophic and frequently experiences cyanobacterial harmful algal blooms (cyanoHABs). In the past, surveys of the phytoplankt...

Descripción completa

Detalles Bibliográficos
Autores principales: Lefler, Forrest W., Barbosa, Maximiliano, Zimba, Paul V., Smyth, Ashley R., Berthold, David E., Laughinghouse, H. Dail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10499181/
https://www.ncbi.nlm.nih.gov/pubmed/37711696
http://dx.doi.org/10.3389/fmicb.2023.1219261
_version_ 1785105652030373888
author Lefler, Forrest W.
Barbosa, Maximiliano
Zimba, Paul V.
Smyth, Ashley R.
Berthold, David E.
Laughinghouse, H. Dail
author_facet Lefler, Forrest W.
Barbosa, Maximiliano
Zimba, Paul V.
Smyth, Ashley R.
Berthold, David E.
Laughinghouse, H. Dail
author_sort Lefler, Forrest W.
collection PubMed
description Lake Okeechobee is a large eutrophic, shallow, subtropical lake in south Florida, United States. Due to decades of nutrient loading and phosphorus rich sediments, the lake is eutrophic and frequently experiences cyanobacterial harmful algal blooms (cyanoHABs). In the past, surveys of the phytoplankton community structure in the lake have been conducted by morphological studies, whereas molecular based studies have been seldom employed. With increased frequency of cyanoHABs in Lake Okeechobee (e.g., 2016 and 2018 Microcystis-dominated blooms), it is imperative to determine the diversity of cyanobacterial taxa that exist within the lake and the limnological parameters that drive bloom-forming genera. A spatiotemporal study of the lake was conducted over the course of 1 year to characterize the (cyano)bacterial community structure, using 16S rRNA metabarcoding, with coincident collection of limnological parameters (e.g., nutrients, water temperature, major ions), and cyanotoxins. The objectives of this study were to elucidate spatiotemporal trends of community structure, identify drivers of community structure, and examine cyanobacteria-bacterial relationships within the lake. Results indicated that cyanobacterial communities within the lake were significantly different between the wet and dry season, but not between periods of nitrogen limitation and co-nutrient limitation. Throughout the year, the lake was primarily dominated by the picocyanobacterium Cyanobium. The bloom-forming genera Cuspidothrix, Dolichospermum, Microcystis, and Raphidiopsis were highly abundant throughout the lake and had disparate nutrient requirements and niches within the lake. Anatoxin-a, microcystins, and nodularins were detected throughout the lake across both seasons. There were no correlated (cyano)bacteria shared between the common bloom-forming cyanobacteria Dolichospermum, Microcystis, and Raphidiopsis. This study is the first of its kind to use molecular based methods to assess the cyanobacterial community structure within the lake. These data greatly improve our understanding of the cyanobacterial community structure within the lake and the physiochemical parameters which may drive the bloom-forming taxa within Lake Okeechobee.
format Online
Article
Text
id pubmed-10499181
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-104991812023-09-14 Spatiotemporal diversity and community structure of cyanobacteria and associated bacteria in the large shallow subtropical Lake Okeechobee (Florida, United States) Lefler, Forrest W. Barbosa, Maximiliano Zimba, Paul V. Smyth, Ashley R. Berthold, David E. Laughinghouse, H. Dail Front Microbiol Microbiology Lake Okeechobee is a large eutrophic, shallow, subtropical lake in south Florida, United States. Due to decades of nutrient loading and phosphorus rich sediments, the lake is eutrophic and frequently experiences cyanobacterial harmful algal blooms (cyanoHABs). In the past, surveys of the phytoplankton community structure in the lake have been conducted by morphological studies, whereas molecular based studies have been seldom employed. With increased frequency of cyanoHABs in Lake Okeechobee (e.g., 2016 and 2018 Microcystis-dominated blooms), it is imperative to determine the diversity of cyanobacterial taxa that exist within the lake and the limnological parameters that drive bloom-forming genera. A spatiotemporal study of the lake was conducted over the course of 1 year to characterize the (cyano)bacterial community structure, using 16S rRNA metabarcoding, with coincident collection of limnological parameters (e.g., nutrients, water temperature, major ions), and cyanotoxins. The objectives of this study were to elucidate spatiotemporal trends of community structure, identify drivers of community structure, and examine cyanobacteria-bacterial relationships within the lake. Results indicated that cyanobacterial communities within the lake were significantly different between the wet and dry season, but not between periods of nitrogen limitation and co-nutrient limitation. Throughout the year, the lake was primarily dominated by the picocyanobacterium Cyanobium. The bloom-forming genera Cuspidothrix, Dolichospermum, Microcystis, and Raphidiopsis were highly abundant throughout the lake and had disparate nutrient requirements and niches within the lake. Anatoxin-a, microcystins, and nodularins were detected throughout the lake across both seasons. There were no correlated (cyano)bacteria shared between the common bloom-forming cyanobacteria Dolichospermum, Microcystis, and Raphidiopsis. This study is the first of its kind to use molecular based methods to assess the cyanobacterial community structure within the lake. These data greatly improve our understanding of the cyanobacterial community structure within the lake and the physiochemical parameters which may drive the bloom-forming taxa within Lake Okeechobee. Frontiers Media S.A. 2023-08-30 /pmc/articles/PMC10499181/ /pubmed/37711696 http://dx.doi.org/10.3389/fmicb.2023.1219261 Text en Copyright © 2023 Lefler, Barbosa, Zimba, Smyth, Berthold and Laughinghouse. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lefler, Forrest W.
Barbosa, Maximiliano
Zimba, Paul V.
Smyth, Ashley R.
Berthold, David E.
Laughinghouse, H. Dail
Spatiotemporal diversity and community structure of cyanobacteria and associated bacteria in the large shallow subtropical Lake Okeechobee (Florida, United States)
title Spatiotemporal diversity and community structure of cyanobacteria and associated bacteria in the large shallow subtropical Lake Okeechobee (Florida, United States)
title_full Spatiotemporal diversity and community structure of cyanobacteria and associated bacteria in the large shallow subtropical Lake Okeechobee (Florida, United States)
title_fullStr Spatiotemporal diversity and community structure of cyanobacteria and associated bacteria in the large shallow subtropical Lake Okeechobee (Florida, United States)
title_full_unstemmed Spatiotemporal diversity and community structure of cyanobacteria and associated bacteria in the large shallow subtropical Lake Okeechobee (Florida, United States)
title_short Spatiotemporal diversity and community structure of cyanobacteria and associated bacteria in the large shallow subtropical Lake Okeechobee (Florida, United States)
title_sort spatiotemporal diversity and community structure of cyanobacteria and associated bacteria in the large shallow subtropical lake okeechobee (florida, united states)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10499181/
https://www.ncbi.nlm.nih.gov/pubmed/37711696
http://dx.doi.org/10.3389/fmicb.2023.1219261
work_keys_str_mv AT leflerforrestw spatiotemporaldiversityandcommunitystructureofcyanobacteriaandassociatedbacteriainthelargeshallowsubtropicallakeokeechobeefloridaunitedstates
AT barbosamaximiliano spatiotemporaldiversityandcommunitystructureofcyanobacteriaandassociatedbacteriainthelargeshallowsubtropicallakeokeechobeefloridaunitedstates
AT zimbapaulv spatiotemporaldiversityandcommunitystructureofcyanobacteriaandassociatedbacteriainthelargeshallowsubtropicallakeokeechobeefloridaunitedstates
AT smythashleyr spatiotemporaldiversityandcommunitystructureofcyanobacteriaandassociatedbacteriainthelargeshallowsubtropicallakeokeechobeefloridaunitedstates
AT bertholddavide spatiotemporaldiversityandcommunitystructureofcyanobacteriaandassociatedbacteriainthelargeshallowsubtropicallakeokeechobeefloridaunitedstates
AT laughinghousehdail spatiotemporaldiversityandcommunitystructureofcyanobacteriaandassociatedbacteriainthelargeshallowsubtropicallakeokeechobeefloridaunitedstates