CRISPR-based engineering of RNA viruses

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CRISPR RNA–guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a recombin...

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Detalles Bibliográficos
Autores principales: Nemudryi, Artem, Nemudraia, Anna, Nichols, Joseph E., Scherffius, Andrew M., Zahl, Trevor, Wiedenheft, Blake
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Biomedicine and Life Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10499312/
https://www.ncbi.nlm.nih.gov/pubmed/37703376
http://dx.doi.org/10.1126/sciadv.adj8277
Descripción
Sumario:CRISPR RNA–guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a recombinant RNA technology with immediate applications for the facile engineering of RNA viruses.

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