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CRISPR-based engineering of RNA viruses
CRISPR RNA–guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a recombin...
Autores principales: | Nemudryi, Artem, Nemudraia, Anna, Nichols, Joseph E., Scherffius, Andrew M., Zahl, Trevor, Wiedenheft, Blake |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10499312/ https://www.ncbi.nlm.nih.gov/pubmed/37703376 http://dx.doi.org/10.1126/sciadv.adj8277 |
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