Cryo-EM structure determination of small therapeutic protein targets at 3 Å-resolution using a rigid imaging scaffold

Cryoelectron microscopy (Cryo-EM) has enabled structural determination of proteins larger than about 50 kDa, including many intractable by any other method, but it has largely failed for smaller proteins. Here, we obtain structures of small proteins by binding them to a rigid molecular scaffold base...

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Detalles Bibliográficos
Autores principales: Castells-Graells, Roger, Meador, Kyle, Arbing, Mark A., Sawaya, Michael R., Gee, Morgan, Cascio, Duilio, Gleave, Emma, Debreczeni, Judit É., Breed, Jason, Leopold, Karoline, Patel, Ankoor, Jahagirdar, Dushyant, Lyons, Bronwyn, Subramaniam, Sriram, Phillips, Chris, Yeates, Todd O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10500258/
https://www.ncbi.nlm.nih.gov/pubmed/37669364
http://dx.doi.org/10.1073/pnas.2305494120

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