Enrichment of carcinogen-driven “mitochondria-primed” human skin stem cells and their identification using single-cell analyses

Previous work has shown that mitochondria play a critical role in priming stem cells to self-renew and proliferate. Here, we describe a protocol for enriching and identifying the mitochondria-primed stem cells (mpSCs) for their characterization and applications. We describe steps for enriching mpSCs...

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Detalles Bibliográficos
Autores principales: Agarwala, Swati, Saini, Mayank, Parker, Danitra, Mitra, Kasturi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10500471/
https://www.ncbi.nlm.nih.gov/pubmed/37690024
http://dx.doi.org/10.1016/j.xpro.2023.102545
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author Agarwala, Swati
Saini, Mayank
Parker, Danitra
Mitra, Kasturi
author_facet Agarwala, Swati
Saini, Mayank
Parker, Danitra
Mitra, Kasturi
author_sort Agarwala, Swati
collection PubMed
description Previous work has shown that mitochondria play a critical role in priming stem cells to self-renew and proliferate. Here, we describe a protocol for enriching and identifying the mitochondria-primed stem cells (mpSCs) for their characterization and applications. We describe steps for enriching mpSCs with the environmental carcinogen 2,3,7,8-tetrachlorodibenzo-p-dioxin in a skin keratinocyte lineage and for identifying mpSCs using single-cell transcriptomics and single-cell microscopy analyses of expression of relevant stem cell markers. For complete details on the use and execution of this protocol, please refer to Spurlock et al.(1)
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spelling pubmed-105004712023-09-15 Enrichment of carcinogen-driven “mitochondria-primed” human skin stem cells and their identification using single-cell analyses Agarwala, Swati Saini, Mayank Parker, Danitra Mitra, Kasturi STAR Protoc Protocol Previous work has shown that mitochondria play a critical role in priming stem cells to self-renew and proliferate. Here, we describe a protocol for enriching and identifying the mitochondria-primed stem cells (mpSCs) for their characterization and applications. We describe steps for enriching mpSCs with the environmental carcinogen 2,3,7,8-tetrachlorodibenzo-p-dioxin in a skin keratinocyte lineage and for identifying mpSCs using single-cell transcriptomics and single-cell microscopy analyses of expression of relevant stem cell markers. For complete details on the use and execution of this protocol, please refer to Spurlock et al.(1) Elsevier 2023-09-09 /pmc/articles/PMC10500471/ /pubmed/37690024 http://dx.doi.org/10.1016/j.xpro.2023.102545 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Agarwala, Swati
Saini, Mayank
Parker, Danitra
Mitra, Kasturi
Enrichment of carcinogen-driven “mitochondria-primed” human skin stem cells and their identification using single-cell analyses
title Enrichment of carcinogen-driven “mitochondria-primed” human skin stem cells and their identification using single-cell analyses
title_full Enrichment of carcinogen-driven “mitochondria-primed” human skin stem cells and their identification using single-cell analyses
title_fullStr Enrichment of carcinogen-driven “mitochondria-primed” human skin stem cells and their identification using single-cell analyses
title_full_unstemmed Enrichment of carcinogen-driven “mitochondria-primed” human skin stem cells and their identification using single-cell analyses
title_short Enrichment of carcinogen-driven “mitochondria-primed” human skin stem cells and their identification using single-cell analyses
title_sort enrichment of carcinogen-driven “mitochondria-primed” human skin stem cells and their identification using single-cell analyses
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10500471/
https://www.ncbi.nlm.nih.gov/pubmed/37690024
http://dx.doi.org/10.1016/j.xpro.2023.102545
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