Detection of Frog Virus 3 by Integrating RPA-CRISPR/Cas12a-SPM with Deep Learning

[Image: see text] A fast, easy-to-implement, highly sensitive, and point-of-care (POC) detection system for frog virus 3 (FV3) is proposed. Combining recombinase polymerase amplification (RPA) and CRISPR/Cas12a, a limit of detection (LoD) of 100 aM (60.2 copies/μL) is achieved by optimizing RPA prim...

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Detalles Bibliográficos
Autores principales: Lei, Zhengyang, Lian, Lijin, Zhang, Likun, Liu, Changyue, Zhai, Shiyao, Yuan, Xi, Wei, Jiazhang, Liu, Hong, Liu, Ying, Du, Zhicheng, Gul, Ijaz, Zhang, Haihui, Qin, Zhifeng, Zeng, Shaoling, Jia, Peng, Du, Ke, Deng, Lin, Yu, Dongmei, He, Qian, Qin, Peiwu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10500685/
https://www.ncbi.nlm.nih.gov/pubmed/37720737
http://dx.doi.org/10.1021/acsomega.3c02929

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