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RNA-seq analysis of gene expression profiles in posttraumatic stress disorder, Parkinson’s disease and schizophrenia identifies roles for common and distinct biological pathways

Evidence suggests that shared pathophysiological mechanisms in neuropsychiatric disorders (NPDs) may contribute to risk and resilience. We used single-gene and network-level transcriptomic approaches to investigate shared and disorder-specific processes underlying posttraumatic stress disorder (PTSD...

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Autores principales: Hemmings, Sian M. J., Swart, Patricia, Womersely, Jacqueline S., Ovenden, Ellen S., van den Heuvel, Leigh L., McGregor, Nathaniel W., Meier, Stuart, Bardien, Soraya, Abrahams, Shameemah, Tromp, Gerard, Emsley, Robin, Carr, Jonathan, Seedat, Soraya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10501040/
https://www.ncbi.nlm.nih.gov/pubmed/37861850
http://dx.doi.org/10.1007/s44192-022-00009-y
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author Hemmings, Sian M. J.
Swart, Patricia
Womersely, Jacqueline S.
Ovenden, Ellen S.
van den Heuvel, Leigh L.
McGregor, Nathaniel W.
Meier, Stuart
Bardien, Soraya
Abrahams, Shameemah
Tromp, Gerard
Emsley, Robin
Carr, Jonathan
Seedat, Soraya
author_facet Hemmings, Sian M. J.
Swart, Patricia
Womersely, Jacqueline S.
Ovenden, Ellen S.
van den Heuvel, Leigh L.
McGregor, Nathaniel W.
Meier, Stuart
Bardien, Soraya
Abrahams, Shameemah
Tromp, Gerard
Emsley, Robin
Carr, Jonathan
Seedat, Soraya
author_sort Hemmings, Sian M. J.
collection PubMed
description Evidence suggests that shared pathophysiological mechanisms in neuropsychiatric disorders (NPDs) may contribute to risk and resilience. We used single-gene and network-level transcriptomic approaches to investigate shared and disorder-specific processes underlying posttraumatic stress disorder (PTSD), Parkinson’s disease (PD) and schizophrenia in a South African sample. RNA-seq was performed on blood obtained from cases and controls from each cohort. Gene expression and weighted gene correlation network analyses (WGCNA) were performed using DESeq2 and CEMiTool, respectively. Significant differences in gene expression were limited to the PTSD cohort. However, WGCNA implicated, amongst others, ribosomal expression, inflammation and ubiquitination as key players in the NPDs under investigation. Differential expression in ribosomal-related pathways was observed in the PTSD and PD cohorts, and focal adhesion and extracellular matrix pathways were implicated in PD and schizophrenia. We propose that, despite different phenotypic presentations, core transdiagnostic mechanisms may play important roles in the molecular aetiology of NPDs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s44192-022-00009-y.
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spelling pubmed-105010402023-10-17 RNA-seq analysis of gene expression profiles in posttraumatic stress disorder, Parkinson’s disease and schizophrenia identifies roles for common and distinct biological pathways Hemmings, Sian M. J. Swart, Patricia Womersely, Jacqueline S. Ovenden, Ellen S. van den Heuvel, Leigh L. McGregor, Nathaniel W. Meier, Stuart Bardien, Soraya Abrahams, Shameemah Tromp, Gerard Emsley, Robin Carr, Jonathan Seedat, Soraya Discov Ment Health Research Evidence suggests that shared pathophysiological mechanisms in neuropsychiatric disorders (NPDs) may contribute to risk and resilience. We used single-gene and network-level transcriptomic approaches to investigate shared and disorder-specific processes underlying posttraumatic stress disorder (PTSD), Parkinson’s disease (PD) and schizophrenia in a South African sample. RNA-seq was performed on blood obtained from cases and controls from each cohort. Gene expression and weighted gene correlation network analyses (WGCNA) were performed using DESeq2 and CEMiTool, respectively. Significant differences in gene expression were limited to the PTSD cohort. However, WGCNA implicated, amongst others, ribosomal expression, inflammation and ubiquitination as key players in the NPDs under investigation. Differential expression in ribosomal-related pathways was observed in the PTSD and PD cohorts, and focal adhesion and extracellular matrix pathways were implicated in PD and schizophrenia. We propose that, despite different phenotypic presentations, core transdiagnostic mechanisms may play important roles in the molecular aetiology of NPDs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s44192-022-00009-y. Springer International Publishing 2022-03-03 /pmc/articles/PMC10501040/ /pubmed/37861850 http://dx.doi.org/10.1007/s44192-022-00009-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Hemmings, Sian M. J.
Swart, Patricia
Womersely, Jacqueline S.
Ovenden, Ellen S.
van den Heuvel, Leigh L.
McGregor, Nathaniel W.
Meier, Stuart
Bardien, Soraya
Abrahams, Shameemah
Tromp, Gerard
Emsley, Robin
Carr, Jonathan
Seedat, Soraya
RNA-seq analysis of gene expression profiles in posttraumatic stress disorder, Parkinson’s disease and schizophrenia identifies roles for common and distinct biological pathways
title RNA-seq analysis of gene expression profiles in posttraumatic stress disorder, Parkinson’s disease and schizophrenia identifies roles for common and distinct biological pathways
title_full RNA-seq analysis of gene expression profiles in posttraumatic stress disorder, Parkinson’s disease and schizophrenia identifies roles for common and distinct biological pathways
title_fullStr RNA-seq analysis of gene expression profiles in posttraumatic stress disorder, Parkinson’s disease and schizophrenia identifies roles for common and distinct biological pathways
title_full_unstemmed RNA-seq analysis of gene expression profiles in posttraumatic stress disorder, Parkinson’s disease and schizophrenia identifies roles for common and distinct biological pathways
title_short RNA-seq analysis of gene expression profiles in posttraumatic stress disorder, Parkinson’s disease and schizophrenia identifies roles for common and distinct biological pathways
title_sort rna-seq analysis of gene expression profiles in posttraumatic stress disorder, parkinson’s disease and schizophrenia identifies roles for common and distinct biological pathways
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10501040/
https://www.ncbi.nlm.nih.gov/pubmed/37861850
http://dx.doi.org/10.1007/s44192-022-00009-y
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