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Characterization of a virulence factor in Plasmodiophora brassicae, with molecular markers for identification

Symptom severity on differential host lines is currently used to characterize and identify pathotypes of Plasmodiophora brassicae, which is an obligate, soil-borne chromist pathogen that causes clubroot disease on canola (Brassica napus) and other brassica crops. This process is slow, variable and r...

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Autores principales: Sedaghatkish, Afsaneh, Gossen, Bruce D., McDonald, Mary Ruth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10501564/
https://www.ncbi.nlm.nih.gov/pubmed/37708170
http://dx.doi.org/10.1371/journal.pone.0289842
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author Sedaghatkish, Afsaneh
Gossen, Bruce D.
McDonald, Mary Ruth
author_facet Sedaghatkish, Afsaneh
Gossen, Bruce D.
McDonald, Mary Ruth
author_sort Sedaghatkish, Afsaneh
collection PubMed
description Symptom severity on differential host lines is currently used to characterize and identify pathotypes of Plasmodiophora brassicae, which is an obligate, soil-borne chromist pathogen that causes clubroot disease on canola (Brassica napus) and other brassica crops. This process is slow, variable and resource intensive; development of molecular markers could make identification of important pathotypes faster and more consistent for deployment of cultivars with pathotype-specific resistance. In the current study, a variant of gene 9171 was identified in the whole-genome sequences of only the highly virulent pathotypes of P. brassicae from around the world, including the new cohort of virulent pathotypes in Canada; its presence was confirmed using three KASP marker pairs. The gene was not present in the initial cohort of pathotypes identified in Canada. The putative structure, domains, and gene ontogeny of the protein product of gene 9171 were assessed using on-line software resources. Structural analysis of the putative protein produced by gene 9171 indicated that it was localized in the cytosol, and likely involved in cellular processes and catalytic activity. Identification of gene 9171 represents a potentially useful step toward molecular identification of the pathotypes of P. brassicae.
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spelling pubmed-105015642023-09-15 Characterization of a virulence factor in Plasmodiophora brassicae, with molecular markers for identification Sedaghatkish, Afsaneh Gossen, Bruce D. McDonald, Mary Ruth PLoS One Research Article Symptom severity on differential host lines is currently used to characterize and identify pathotypes of Plasmodiophora brassicae, which is an obligate, soil-borne chromist pathogen that causes clubroot disease on canola (Brassica napus) and other brassica crops. This process is slow, variable and resource intensive; development of molecular markers could make identification of important pathotypes faster and more consistent for deployment of cultivars with pathotype-specific resistance. In the current study, a variant of gene 9171 was identified in the whole-genome sequences of only the highly virulent pathotypes of P. brassicae from around the world, including the new cohort of virulent pathotypes in Canada; its presence was confirmed using three KASP marker pairs. The gene was not present in the initial cohort of pathotypes identified in Canada. The putative structure, domains, and gene ontogeny of the protein product of gene 9171 were assessed using on-line software resources. Structural analysis of the putative protein produced by gene 9171 indicated that it was localized in the cytosol, and likely involved in cellular processes and catalytic activity. Identification of gene 9171 represents a potentially useful step toward molecular identification of the pathotypes of P. brassicae. Public Library of Science 2023-09-14 /pmc/articles/PMC10501564/ /pubmed/37708170 http://dx.doi.org/10.1371/journal.pone.0289842 Text en © 2023 Sedaghatkish et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sedaghatkish, Afsaneh
Gossen, Bruce D.
McDonald, Mary Ruth
Characterization of a virulence factor in Plasmodiophora brassicae, with molecular markers for identification
title Characterization of a virulence factor in Plasmodiophora brassicae, with molecular markers for identification
title_full Characterization of a virulence factor in Plasmodiophora brassicae, with molecular markers for identification
title_fullStr Characterization of a virulence factor in Plasmodiophora brassicae, with molecular markers for identification
title_full_unstemmed Characterization of a virulence factor in Plasmodiophora brassicae, with molecular markers for identification
title_short Characterization of a virulence factor in Plasmodiophora brassicae, with molecular markers for identification
title_sort characterization of a virulence factor in plasmodiophora brassicae, with molecular markers for identification
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10501564/
https://www.ncbi.nlm.nih.gov/pubmed/37708170
http://dx.doi.org/10.1371/journal.pone.0289842
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