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Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation

Understanding of the molecular drivers of lineage diversification and tissue patterning during primary germ layer development requires in-depth knowledge of the dynamic molecular trajectories of cell lineages across a series of developmental stages of gastrulation. Through computational modeling, we...

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Autores principales: Wang, Ran, Yang, Xianfa, Chen, Jiehui, Zhang, Lin, Griffiths, Jonathan A., Cui, Guizhong, Chen, Yingying, Qian, Yun, Peng, Guangdun, Li, Jinsong, Wang, Liantang, Marioni, John C., Tam, Patrick P. L., Jing, Naihe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10502153/
https://www.ncbi.nlm.nih.gov/pubmed/37709743
http://dx.doi.org/10.1038/s41467-023-41482-5
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author Wang, Ran
Yang, Xianfa
Chen, Jiehui
Zhang, Lin
Griffiths, Jonathan A.
Cui, Guizhong
Chen, Yingying
Qian, Yun
Peng, Guangdun
Li, Jinsong
Wang, Liantang
Marioni, John C.
Tam, Patrick P. L.
Jing, Naihe
author_facet Wang, Ran
Yang, Xianfa
Chen, Jiehui
Zhang, Lin
Griffiths, Jonathan A.
Cui, Guizhong
Chen, Yingying
Qian, Yun
Peng, Guangdun
Li, Jinsong
Wang, Liantang
Marioni, John C.
Tam, Patrick P. L.
Jing, Naihe
author_sort Wang, Ran
collection PubMed
description Understanding of the molecular drivers of lineage diversification and tissue patterning during primary germ layer development requires in-depth knowledge of the dynamic molecular trajectories of cell lineages across a series of developmental stages of gastrulation. Through computational modeling, we constructed at single-cell resolution, a spatio-temporal transcriptome of cell populations in the germ-layers of gastrula-stage mouse embryos. This molecular atlas enables the inference of molecular network activity underpinning the specification and differentiation of the germ-layer tissue lineages. Heterogeneity analysis of cellular composition at defined positions in the epiblast revealed progressive diversification of cell types. The single-cell transcriptome revealed an enhanced BMP signaling activity in the right-side mesoderm of late-gastrulation embryo. Perturbation of asymmetric BMP signaling activity at late gastrulation led to randomization of left-right molecular asymmetry in the lateral mesoderm of early-somite-stage embryo. These findings indicate the asymmetric BMP activity during gastrulation may be critical for the symmetry breaking process.
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spelling pubmed-105021532023-09-16 Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation Wang, Ran Yang, Xianfa Chen, Jiehui Zhang, Lin Griffiths, Jonathan A. Cui, Guizhong Chen, Yingying Qian, Yun Peng, Guangdun Li, Jinsong Wang, Liantang Marioni, John C. Tam, Patrick P. L. Jing, Naihe Nat Commun Article Understanding of the molecular drivers of lineage diversification and tissue patterning during primary germ layer development requires in-depth knowledge of the dynamic molecular trajectories of cell lineages across a series of developmental stages of gastrulation. Through computational modeling, we constructed at single-cell resolution, a spatio-temporal transcriptome of cell populations in the germ-layers of gastrula-stage mouse embryos. This molecular atlas enables the inference of molecular network activity underpinning the specification and differentiation of the germ-layer tissue lineages. Heterogeneity analysis of cellular composition at defined positions in the epiblast revealed progressive diversification of cell types. The single-cell transcriptome revealed an enhanced BMP signaling activity in the right-side mesoderm of late-gastrulation embryo. Perturbation of asymmetric BMP signaling activity at late gastrulation led to randomization of left-right molecular asymmetry in the lateral mesoderm of early-somite-stage embryo. These findings indicate the asymmetric BMP activity during gastrulation may be critical for the symmetry breaking process. Nature Publishing Group UK 2023-09-14 /pmc/articles/PMC10502153/ /pubmed/37709743 http://dx.doi.org/10.1038/s41467-023-41482-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wang, Ran
Yang, Xianfa
Chen, Jiehui
Zhang, Lin
Griffiths, Jonathan A.
Cui, Guizhong
Chen, Yingying
Qian, Yun
Peng, Guangdun
Li, Jinsong
Wang, Liantang
Marioni, John C.
Tam, Patrick P. L.
Jing, Naihe
Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation
title Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation
title_full Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation
title_fullStr Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation
title_full_unstemmed Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation
title_short Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation
title_sort time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10502153/
https://www.ncbi.nlm.nih.gov/pubmed/37709743
http://dx.doi.org/10.1038/s41467-023-41482-5
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