Attachment culture of cortical organoids at the microwell air-liquid interface

The cortical organoid is an efficient model for studying human brain neurodevelopment and neurological disease. However, its three-dimensional structure limits real-time observation of internal physiological changes. Here, we present a protocol for an air-liquid interface attachment culture for cort...

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Detalles Bibliográficos
Autores principales: Tang, Jiyuan, Zheng, Honghui, Feng, Yilin, Zeng, Junhong, Ma, Shaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10502426/
https://www.ncbi.nlm.nih.gov/pubmed/37715950
http://dx.doi.org/10.1016/j.xpro.2023.102502
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author Tang, Jiyuan
Zheng, Honghui
Feng, Yilin
Zeng, Junhong
Ma, Shaohua
author_facet Tang, Jiyuan
Zheng, Honghui
Feng, Yilin
Zeng, Junhong
Ma, Shaohua
author_sort Tang, Jiyuan
collection PubMed
description The cortical organoid is an efficient model for studying human brain neurodevelopment and neurological disease. However, its three-dimensional structure limits real-time observation of internal physiological changes. Here, we present a protocol for an air-liquid interface attachment culture for cortical organoids. We describe steps for transplanting cortical organoid slices and generating the air-liquid interface. We then detail calcium imaging on organoid external neural networks and immunohistochemical staining on confocal plates.
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spelling pubmed-105024262023-09-16 Attachment culture of cortical organoids at the microwell air-liquid interface Tang, Jiyuan Zheng, Honghui Feng, Yilin Zeng, Junhong Ma, Shaohua STAR Protoc Protocol The cortical organoid is an efficient model for studying human brain neurodevelopment and neurological disease. However, its three-dimensional structure limits real-time observation of internal physiological changes. Here, we present a protocol for an air-liquid interface attachment culture for cortical organoids. We describe steps for transplanting cortical organoid slices and generating the air-liquid interface. We then detail calcium imaging on organoid external neural networks and immunohistochemical staining on confocal plates. Elsevier 2023-09-12 /pmc/articles/PMC10502426/ /pubmed/37715950 http://dx.doi.org/10.1016/j.xpro.2023.102502 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Tang, Jiyuan
Zheng, Honghui
Feng, Yilin
Zeng, Junhong
Ma, Shaohua
Attachment culture of cortical organoids at the microwell air-liquid interface
title Attachment culture of cortical organoids at the microwell air-liquid interface
title_full Attachment culture of cortical organoids at the microwell air-liquid interface
title_fullStr Attachment culture of cortical organoids at the microwell air-liquid interface
title_full_unstemmed Attachment culture of cortical organoids at the microwell air-liquid interface
title_short Attachment culture of cortical organoids at the microwell air-liquid interface
title_sort attachment culture of cortical organoids at the microwell air-liquid interface
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10502426/
https://www.ncbi.nlm.nih.gov/pubmed/37715950
http://dx.doi.org/10.1016/j.xpro.2023.102502
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AT fengyilin attachmentcultureofcorticalorganoidsatthemicrowellairliquidinterface
AT zengjunhong attachmentcultureofcorticalorganoidsatthemicrowellairliquidinterface
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