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MdNAC104 positively regulates apple cold tolerance via CBF‐dependent and CBF‐independent pathways
Low temperature is the main environmental factor affecting the yield, quality and geographical distribution of crops, which significantly restricts development of the fruit industry. The NAC (NAM, ATAF1/2 and CUC2) transcription factor (TF) family is involved in regulating plant cold tolerance, but...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10502760/ https://www.ncbi.nlm.nih.gov/pubmed/37387580 http://dx.doi.org/10.1111/pbi.14112 |
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author | Mei, Chuang Yang, Jie Mei, Quanlin Jia, Dongfeng Yan, Peng Feng, Beibei Mamat, Aisajan Gong, Xiaoqing Guan, Qingmei Mao, Ke Wang, Jixun Ma, Fengwang |
author_facet | Mei, Chuang Yang, Jie Mei, Quanlin Jia, Dongfeng Yan, Peng Feng, Beibei Mamat, Aisajan Gong, Xiaoqing Guan, Qingmei Mao, Ke Wang, Jixun Ma, Fengwang |
author_sort | Mei, Chuang |
collection | PubMed |
description | Low temperature is the main environmental factor affecting the yield, quality and geographical distribution of crops, which significantly restricts development of the fruit industry. The NAC (NAM, ATAF1/2 and CUC2) transcription factor (TF) family is involved in regulating plant cold tolerance, but the mechanisms underlying these regulatory processes remain unclear. Here, the NAC TF MdNAC104 played a positive role in modulating apple cold tolerance. Under cold stress, MdNAC104‐overexpressing transgenic plants exhibited less ion leakage and lower ROS (reactive oxygen species) accumulation, but higher contents of osmoregulatory substances and activities of antioxidant enzymes. Transcriptional regulation analysis showed that MdNAC104 directly bound to the MdCBF1 and MdCBF3 promoters to promote expression. In addition, based on combined transcriptomic and metabolomic analyses, as well as promoter binding and transcriptional regulation analyses, we found that MdNAC104 stimulated the accumulation of anthocyanin under cold conditions by upregulating the expression of anthocyanin synthesis‐related genes, including MdCHS‐b, MdCHI‐a, MdF3H‐a and MdANS‐b, and increased the activities of the antioxidant enzymes by promoting the expression of the antioxidant enzyme‐encoding genes MdFSD2 and MdPRXR1.1. In conclusion, this study revealed the MdNAC104 regulatory mechanism of cold tolerance in apple via CBF‐dependent and CBF‐independent pathways. |
format | Online Article Text |
id | pubmed-10502760 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105027602023-09-16 MdNAC104 positively regulates apple cold tolerance via CBF‐dependent and CBF‐independent pathways Mei, Chuang Yang, Jie Mei, Quanlin Jia, Dongfeng Yan, Peng Feng, Beibei Mamat, Aisajan Gong, Xiaoqing Guan, Qingmei Mao, Ke Wang, Jixun Ma, Fengwang Plant Biotechnol J Research Articles Low temperature is the main environmental factor affecting the yield, quality and geographical distribution of crops, which significantly restricts development of the fruit industry. The NAC (NAM, ATAF1/2 and CUC2) transcription factor (TF) family is involved in regulating plant cold tolerance, but the mechanisms underlying these regulatory processes remain unclear. Here, the NAC TF MdNAC104 played a positive role in modulating apple cold tolerance. Under cold stress, MdNAC104‐overexpressing transgenic plants exhibited less ion leakage and lower ROS (reactive oxygen species) accumulation, but higher contents of osmoregulatory substances and activities of antioxidant enzymes. Transcriptional regulation analysis showed that MdNAC104 directly bound to the MdCBF1 and MdCBF3 promoters to promote expression. In addition, based on combined transcriptomic and metabolomic analyses, as well as promoter binding and transcriptional regulation analyses, we found that MdNAC104 stimulated the accumulation of anthocyanin under cold conditions by upregulating the expression of anthocyanin synthesis‐related genes, including MdCHS‐b, MdCHI‐a, MdF3H‐a and MdANS‐b, and increased the activities of the antioxidant enzymes by promoting the expression of the antioxidant enzyme‐encoding genes MdFSD2 and MdPRXR1.1. In conclusion, this study revealed the MdNAC104 regulatory mechanism of cold tolerance in apple via CBF‐dependent and CBF‐independent pathways. John Wiley and Sons Inc. 2023-06-30 2023-10 /pmc/articles/PMC10502760/ /pubmed/37387580 http://dx.doi.org/10.1111/pbi.14112 Text en © 2023 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Mei, Chuang Yang, Jie Mei, Quanlin Jia, Dongfeng Yan, Peng Feng, Beibei Mamat, Aisajan Gong, Xiaoqing Guan, Qingmei Mao, Ke Wang, Jixun Ma, Fengwang MdNAC104 positively regulates apple cold tolerance via CBF‐dependent and CBF‐independent pathways |
title | MdNAC104 positively regulates apple cold tolerance via CBF‐dependent and CBF‐independent pathways |
title_full | MdNAC104 positively regulates apple cold tolerance via CBF‐dependent and CBF‐independent pathways |
title_fullStr | MdNAC104 positively regulates apple cold tolerance via CBF‐dependent and CBF‐independent pathways |
title_full_unstemmed | MdNAC104 positively regulates apple cold tolerance via CBF‐dependent and CBF‐independent pathways |
title_short | MdNAC104 positively regulates apple cold tolerance via CBF‐dependent and CBF‐independent pathways |
title_sort | mdnac104 positively regulates apple cold tolerance via cbf‐dependent and cbf‐independent pathways |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10502760/ https://www.ncbi.nlm.nih.gov/pubmed/37387580 http://dx.doi.org/10.1111/pbi.14112 |
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