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Human parvovirus B19 infection in malignant and benign tissue specimens of different head and neck anatomical subsites
BACKGROUND: The role of human parvovirus B19 (B19V) infection in malignant and benign lesions such as head and neck squamous cell carcinomas (HNSCCs) and oral mucocele lesions has not been established. Herein, we examined, for the first time, the presence of B19V in HNSCCs from Iranian subjects. MET...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10503082/ https://www.ncbi.nlm.nih.gov/pubmed/37710342 http://dx.doi.org/10.1186/s13027-023-00528-5 |
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author | Abuei, Haniyeh Namdari, Sepide Pakdel, Tahereh Pakdel, Fatemeh Andishe-Tadbir, Azadeh Behzad-Behbahani, Abbas Ashraf, Mohammad J. Alavi, Parnian Farhadi, Ali |
author_facet | Abuei, Haniyeh Namdari, Sepide Pakdel, Tahereh Pakdel, Fatemeh Andishe-Tadbir, Azadeh Behzad-Behbahani, Abbas Ashraf, Mohammad J. Alavi, Parnian Farhadi, Ali |
author_sort | Abuei, Haniyeh |
collection | PubMed |
description | BACKGROUND: The role of human parvovirus B19 (B19V) infection in malignant and benign lesions such as head and neck squamous cell carcinomas (HNSCCs) and oral mucocele lesions has not been established. Herein, we examined, for the first time, the presence of B19V in HNSCCs from Iranian subjects. METHODS: One hundred and eight HNSCC specimens were analyzed for the presence of B19V using nested polymerase chain reaction (nPCR) and TaqMan quantitative PCR assays. Immunohistochemistry procedures were performed to evaluate the expression of B19V VP1/VP2 proteins, p16INK4a, and NF-κB in tumor tissues and their adjacent non-tumor tissues. In addition, 40 oral mucocele, 30 oral buccal mucosa swabs, and 30 nasopharyngeal swabs obtained from healthy adults were analyzed as controls. RESULTS: B19V DNA was detected in 36.1% of HNSCCs. Further, 23.3% of HNSCC specimens showed immunoreactivity against B19V VP1/VP2 proteins. There was a significant difference in the frequency of B19V DNA-positive cases between the patient and control groups (p < 0.0001). Moreover, comparing tumoral tissues and their adjacent non-tumor tissues in terms of immunoreactivity against B19V structural proteins, a significant association was found between tumor tissues and B19V infection (p < 0.0001). Finally, investigating the simultaneous presence of B19V and high-risk human papillomaviruses (HPV) DNA, we found a significant association between these two viral infections in HNSCCs (p = 0.031). CONCLUSIONS: To sum up, B19V was frequently present in HNSCC tissues of Iranian patients but mostly absent in the adjacent non-tumor tissues as well as oral mucocele lesions, buccal, and nasopharyngeal swabs of healthy subjects. HPV possibly contributes to B19V persistence in HNSCC tissues. Additional research is required to investigate potential etiological or cofactor roles of B19V in the development of HNSCCs. |
format | Online Article Text |
id | pubmed-10503082 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-105030822023-09-16 Human parvovirus B19 infection in malignant and benign tissue specimens of different head and neck anatomical subsites Abuei, Haniyeh Namdari, Sepide Pakdel, Tahereh Pakdel, Fatemeh Andishe-Tadbir, Azadeh Behzad-Behbahani, Abbas Ashraf, Mohammad J. Alavi, Parnian Farhadi, Ali Infect Agent Cancer Research BACKGROUND: The role of human parvovirus B19 (B19V) infection in malignant and benign lesions such as head and neck squamous cell carcinomas (HNSCCs) and oral mucocele lesions has not been established. Herein, we examined, for the first time, the presence of B19V in HNSCCs from Iranian subjects. METHODS: One hundred and eight HNSCC specimens were analyzed for the presence of B19V using nested polymerase chain reaction (nPCR) and TaqMan quantitative PCR assays. Immunohistochemistry procedures were performed to evaluate the expression of B19V VP1/VP2 proteins, p16INK4a, and NF-κB in tumor tissues and their adjacent non-tumor tissues. In addition, 40 oral mucocele, 30 oral buccal mucosa swabs, and 30 nasopharyngeal swabs obtained from healthy adults were analyzed as controls. RESULTS: B19V DNA was detected in 36.1% of HNSCCs. Further, 23.3% of HNSCC specimens showed immunoreactivity against B19V VP1/VP2 proteins. There was a significant difference in the frequency of B19V DNA-positive cases between the patient and control groups (p < 0.0001). Moreover, comparing tumoral tissues and their adjacent non-tumor tissues in terms of immunoreactivity against B19V structural proteins, a significant association was found between tumor tissues and B19V infection (p < 0.0001). Finally, investigating the simultaneous presence of B19V and high-risk human papillomaviruses (HPV) DNA, we found a significant association between these two viral infections in HNSCCs (p = 0.031). CONCLUSIONS: To sum up, B19V was frequently present in HNSCC tissues of Iranian patients but mostly absent in the adjacent non-tumor tissues as well as oral mucocele lesions, buccal, and nasopharyngeal swabs of healthy subjects. HPV possibly contributes to B19V persistence in HNSCC tissues. Additional research is required to investigate potential etiological or cofactor roles of B19V in the development of HNSCCs. BioMed Central 2023-09-14 /pmc/articles/PMC10503082/ /pubmed/37710342 http://dx.doi.org/10.1186/s13027-023-00528-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Abuei, Haniyeh Namdari, Sepide Pakdel, Tahereh Pakdel, Fatemeh Andishe-Tadbir, Azadeh Behzad-Behbahani, Abbas Ashraf, Mohammad J. Alavi, Parnian Farhadi, Ali Human parvovirus B19 infection in malignant and benign tissue specimens of different head and neck anatomical subsites |
title | Human parvovirus B19 infection in malignant and benign tissue specimens of different head and neck anatomical subsites |
title_full | Human parvovirus B19 infection in malignant and benign tissue specimens of different head and neck anatomical subsites |
title_fullStr | Human parvovirus B19 infection in malignant and benign tissue specimens of different head and neck anatomical subsites |
title_full_unstemmed | Human parvovirus B19 infection in malignant and benign tissue specimens of different head and neck anatomical subsites |
title_short | Human parvovirus B19 infection in malignant and benign tissue specimens of different head and neck anatomical subsites |
title_sort | human parvovirus b19 infection in malignant and benign tissue specimens of different head and neck anatomical subsites |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10503082/ https://www.ncbi.nlm.nih.gov/pubmed/37710342 http://dx.doi.org/10.1186/s13027-023-00528-5 |
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