Cargando…
One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo
Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease. Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease. However, transplanted cells can be inju...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10503631/ https://www.ncbi.nlm.nih.gov/pubmed/37488911 http://dx.doi.org/10.4103/1673-5374.377412 |
_version_ | 1785106563283812352 |
---|---|
author | Feng, Lin Li, Da Tian, Yao Zhao, Chengshun Sun, Yun Kou, Xiaolong Wu, Jun Wang, Liu Gu, Qi Li, Wei Hao, Jie Hu, Baoyang Wang, Yukai |
author_facet | Feng, Lin Li, Da Tian, Yao Zhao, Chengshun Sun, Yun Kou, Xiaolong Wu, Jun Wang, Liu Gu, Qi Li, Wei Hao, Jie Hu, Baoyang Wang, Yukai |
author_sort | Feng, Lin |
collection | PubMed |
description | Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease. Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease. However, transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche. Here, we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells. These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion, effectively maintaining axonal integrity in vitro. Importantly, midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts. Overall, our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation. |
format | Online Article Text |
id | pubmed-10503631 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-105036312023-09-16 One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo Feng, Lin Li, Da Tian, Yao Zhao, Chengshun Sun, Yun Kou, Xiaolong Wu, Jun Wang, Liu Gu, Qi Li, Wei Hao, Jie Hu, Baoyang Wang, Yukai Neural Regen Res Research Article Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease. Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease. However, transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche. Here, we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells. These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion, effectively maintaining axonal integrity in vitro. Importantly, midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts. Overall, our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation. Wolters Kluwer - Medknow 2023-05-31 /pmc/articles/PMC10503631/ /pubmed/37488911 http://dx.doi.org/10.4103/1673-5374.377412 Text en Copyright: © Neural Regeneration Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Research Article Feng, Lin Li, Da Tian, Yao Zhao, Chengshun Sun, Yun Kou, Xiaolong Wu, Jun Wang, Liu Gu, Qi Li, Wei Hao, Jie Hu, Baoyang Wang, Yukai One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo |
title | One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo |
title_full | One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo |
title_fullStr | One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo |
title_full_unstemmed | One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo |
title_short | One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo |
title_sort | one-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10503631/ https://www.ncbi.nlm.nih.gov/pubmed/37488911 http://dx.doi.org/10.4103/1673-5374.377412 |
work_keys_str_mv | AT fenglin onestepcellbiomanufacturingplatformporousgelatinmicrocarrierbeadspromotehumanembryonicstemcellderivedmidbraindopaminergicprogenitorcelldifferentiationinvitroandsurvivalaftertransplantationinvivo AT lida onestepcellbiomanufacturingplatformporousgelatinmicrocarrierbeadspromotehumanembryonicstemcellderivedmidbraindopaminergicprogenitorcelldifferentiationinvitroandsurvivalaftertransplantationinvivo AT tianyao onestepcellbiomanufacturingplatformporousgelatinmicrocarrierbeadspromotehumanembryonicstemcellderivedmidbraindopaminergicprogenitorcelldifferentiationinvitroandsurvivalaftertransplantationinvivo AT zhaochengshun onestepcellbiomanufacturingplatformporousgelatinmicrocarrierbeadspromotehumanembryonicstemcellderivedmidbraindopaminergicprogenitorcelldifferentiationinvitroandsurvivalaftertransplantationinvivo AT sunyun onestepcellbiomanufacturingplatformporousgelatinmicrocarrierbeadspromotehumanembryonicstemcellderivedmidbraindopaminergicprogenitorcelldifferentiationinvitroandsurvivalaftertransplantationinvivo AT kouxiaolong onestepcellbiomanufacturingplatformporousgelatinmicrocarrierbeadspromotehumanembryonicstemcellderivedmidbraindopaminergicprogenitorcelldifferentiationinvitroandsurvivalaftertransplantationinvivo AT wujun onestepcellbiomanufacturingplatformporousgelatinmicrocarrierbeadspromotehumanembryonicstemcellderivedmidbraindopaminergicprogenitorcelldifferentiationinvitroandsurvivalaftertransplantationinvivo AT wangliu onestepcellbiomanufacturingplatformporousgelatinmicrocarrierbeadspromotehumanembryonicstemcellderivedmidbraindopaminergicprogenitorcelldifferentiationinvitroandsurvivalaftertransplantationinvivo AT guqi onestepcellbiomanufacturingplatformporousgelatinmicrocarrierbeadspromotehumanembryonicstemcellderivedmidbraindopaminergicprogenitorcelldifferentiationinvitroandsurvivalaftertransplantationinvivo AT liwei onestepcellbiomanufacturingplatformporousgelatinmicrocarrierbeadspromotehumanembryonicstemcellderivedmidbraindopaminergicprogenitorcelldifferentiationinvitroandsurvivalaftertransplantationinvivo AT haojie onestepcellbiomanufacturingplatformporousgelatinmicrocarrierbeadspromotehumanembryonicstemcellderivedmidbraindopaminergicprogenitorcelldifferentiationinvitroandsurvivalaftertransplantationinvivo AT hubaoyang onestepcellbiomanufacturingplatformporousgelatinmicrocarrierbeadspromotehumanembryonicstemcellderivedmidbraindopaminergicprogenitorcelldifferentiationinvitroandsurvivalaftertransplantationinvivo AT wangyukai onestepcellbiomanufacturingplatformporousgelatinmicrocarrierbeadspromotehumanembryonicstemcellderivedmidbraindopaminergicprogenitorcelldifferentiationinvitroandsurvivalaftertransplantationinvivo |