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One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo

Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease. Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease. However, transplanted cells can be inju...

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Autores principales: Feng, Lin, Li, Da, Tian, Yao, Zhao, Chengshun, Sun, Yun, Kou, Xiaolong, Wu, Jun, Wang, Liu, Gu, Qi, Li, Wei, Hao, Jie, Hu, Baoyang, Wang, Yukai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10503631/
https://www.ncbi.nlm.nih.gov/pubmed/37488911
http://dx.doi.org/10.4103/1673-5374.377412
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author Feng, Lin
Li, Da
Tian, Yao
Zhao, Chengshun
Sun, Yun
Kou, Xiaolong
Wu, Jun
Wang, Liu
Gu, Qi
Li, Wei
Hao, Jie
Hu, Baoyang
Wang, Yukai
author_facet Feng, Lin
Li, Da
Tian, Yao
Zhao, Chengshun
Sun, Yun
Kou, Xiaolong
Wu, Jun
Wang, Liu
Gu, Qi
Li, Wei
Hao, Jie
Hu, Baoyang
Wang, Yukai
author_sort Feng, Lin
collection PubMed
description Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease. Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease. However, transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche. Here, we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells. These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion, effectively maintaining axonal integrity in vitro. Importantly, midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts. Overall, our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation.
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spelling pubmed-105036312023-09-16 One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo Feng, Lin Li, Da Tian, Yao Zhao, Chengshun Sun, Yun Kou, Xiaolong Wu, Jun Wang, Liu Gu, Qi Li, Wei Hao, Jie Hu, Baoyang Wang, Yukai Neural Regen Res Research Article Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease. Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease. However, transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche. Here, we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells. These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion, effectively maintaining axonal integrity in vitro. Importantly, midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts. Overall, our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation. Wolters Kluwer - Medknow 2023-05-31 /pmc/articles/PMC10503631/ /pubmed/37488911 http://dx.doi.org/10.4103/1673-5374.377412 Text en Copyright: © Neural Regeneration Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Research Article
Feng, Lin
Li, Da
Tian, Yao
Zhao, Chengshun
Sun, Yun
Kou, Xiaolong
Wu, Jun
Wang, Liu
Gu, Qi
Li, Wei
Hao, Jie
Hu, Baoyang
Wang, Yukai
One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo
title One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo
title_full One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo
title_fullStr One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo
title_full_unstemmed One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo
title_short One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo
title_sort one-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10503631/
https://www.ncbi.nlm.nih.gov/pubmed/37488911
http://dx.doi.org/10.4103/1673-5374.377412
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