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Cloning, expression, and molecular modification of glycoside hydrolase family 5 genes from Thermoascus aurantiacus
In this paper, a novel bifunctional cellulase gene cel1 was cloned from Thermoascus aurantiacus by PCR and heterologously expressed in Pichia pastoris GS115. Bioinformatics and other related tools were used to compare the nucleotide homology of target genes, and analyze the signal peptide, transmemb...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10503741/ https://www.ncbi.nlm.nih.gov/pubmed/37713448 http://dx.doi.org/10.1371/journal.pone.0285680 |