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Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays
Epilepsy severely impairs the cognitive behavior of patients. It remains unclear whether epilepsy-induced cognitive impairment is associated with neuronal activities in the medial entorhinal cortex (MEC), a region known for its involvement in spatial cognition. To explore this neural mechanism, we r...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AAAS
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10503993/ https://www.ncbi.nlm.nih.gov/pubmed/37719050 http://dx.doi.org/10.34133/research.0229 |
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author | Xu, Zhaojie Mo, Fan Yang, Gucheng Fan, Penghui Lu, Botao Liang, Wei Kong, Fanli Jing, Luyi Xu, Wei Liu, Juntao Wang, Mixia Wu, Yirong Cai, Xinxia |
author_facet | Xu, Zhaojie Mo, Fan Yang, Gucheng Fan, Penghui Lu, Botao Liang, Wei Kong, Fanli Jing, Luyi Xu, Wei Liu, Juntao Wang, Mixia Wu, Yirong Cai, Xinxia |
author_sort | Xu, Zhaojie |
collection | PubMed |
description | Epilepsy severely impairs the cognitive behavior of patients. It remains unclear whether epilepsy-induced cognitive impairment is associated with neuronal activities in the medial entorhinal cortex (MEC), a region known for its involvement in spatial cognition. To explore this neural mechanism, we recorded the spikes and local field potentials from MEC neurons in lithium–pilocarpine-induced epileptic rats using self-designed microelectrode arrays. Through the open field test, we identified spatial cells exhibiting spatially selective firing properties and assessed their spatial representations in relation to the progression of epilepsy. Meanwhile, we analyzed theta oscillations and theta modulation in both excitatory and inhibitory neurons. Furthermore, we used a novel object recognition test to evaluate changes in spatial cognitive ability of epileptic rats. After the epilepsy modeling, the spatial tuning of various types of spatial cells had suffered a rapid and pronounced damage during the latent period (1 to 5 d). Subsequently, the firing characteristics and theta oscillations were impaired. In the chronic period (>10 d), the performance in the novel object experiment deteriorated. In conclusion, our study demonstrates the detrimental effect on spatial representations and electrophysiological properties of MEC neurons in the epileptic latency, suggesting the potential use of these changes as a “functional biomarker” for predicting cognitive impairment caused by epilepsy. |
format | Online Article Text |
id | pubmed-10503993 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | AAAS |
record_format | MEDLINE/PubMed |
spelling | pubmed-105039932023-09-16 Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays Xu, Zhaojie Mo, Fan Yang, Gucheng Fan, Penghui Lu, Botao Liang, Wei Kong, Fanli Jing, Luyi Xu, Wei Liu, Juntao Wang, Mixia Wu, Yirong Cai, Xinxia Research (Wash D C) Research Article Epilepsy severely impairs the cognitive behavior of patients. It remains unclear whether epilepsy-induced cognitive impairment is associated with neuronal activities in the medial entorhinal cortex (MEC), a region known for its involvement in spatial cognition. To explore this neural mechanism, we recorded the spikes and local field potentials from MEC neurons in lithium–pilocarpine-induced epileptic rats using self-designed microelectrode arrays. Through the open field test, we identified spatial cells exhibiting spatially selective firing properties and assessed their spatial representations in relation to the progression of epilepsy. Meanwhile, we analyzed theta oscillations and theta modulation in both excitatory and inhibitory neurons. Furthermore, we used a novel object recognition test to evaluate changes in spatial cognitive ability of epileptic rats. After the epilepsy modeling, the spatial tuning of various types of spatial cells had suffered a rapid and pronounced damage during the latent period (1 to 5 d). Subsequently, the firing characteristics and theta oscillations were impaired. In the chronic period (>10 d), the performance in the novel object experiment deteriorated. In conclusion, our study demonstrates the detrimental effect on spatial representations and electrophysiological properties of MEC neurons in the epileptic latency, suggesting the potential use of these changes as a “functional biomarker” for predicting cognitive impairment caused by epilepsy. AAAS 2023-09-15 /pmc/articles/PMC10503993/ /pubmed/37719050 http://dx.doi.org/10.34133/research.0229 Text en Copyright © 2023 Zhaojie Xu et al. https://creativecommons.org/licenses/by/4.0/Exclusive licensee Science and Technology Review Publishing House. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Xu, Zhaojie Mo, Fan Yang, Gucheng Fan, Penghui Lu, Botao Liang, Wei Kong, Fanli Jing, Luyi Xu, Wei Liu, Juntao Wang, Mixia Wu, Yirong Cai, Xinxia Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays |
title | Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays |
title_full | Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays |
title_fullStr | Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays |
title_full_unstemmed | Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays |
title_short | Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays |
title_sort | impaired spatial firing representations of neurons in the medial entorhinal cortex of the epileptic rat using microelectrode arrays |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10503993/ https://www.ncbi.nlm.nih.gov/pubmed/37719050 http://dx.doi.org/10.34133/research.0229 |
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