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Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays

Epilepsy severely impairs the cognitive behavior of patients. It remains unclear whether epilepsy-induced cognitive impairment is associated with neuronal activities in the medial entorhinal cortex (MEC), a region known for its involvement in spatial cognition. To explore this neural mechanism, we r...

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Autores principales: Xu, Zhaojie, Mo, Fan, Yang, Gucheng, Fan, Penghui, Lu, Botao, Liang, Wei, Kong, Fanli, Jing, Luyi, Xu, Wei, Liu, Juntao, Wang, Mixia, Wu, Yirong, Cai, Xinxia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AAAS 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10503993/
https://www.ncbi.nlm.nih.gov/pubmed/37719050
http://dx.doi.org/10.34133/research.0229
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author Xu, Zhaojie
Mo, Fan
Yang, Gucheng
Fan, Penghui
Lu, Botao
Liang, Wei
Kong, Fanli
Jing, Luyi
Xu, Wei
Liu, Juntao
Wang, Mixia
Wu, Yirong
Cai, Xinxia
author_facet Xu, Zhaojie
Mo, Fan
Yang, Gucheng
Fan, Penghui
Lu, Botao
Liang, Wei
Kong, Fanli
Jing, Luyi
Xu, Wei
Liu, Juntao
Wang, Mixia
Wu, Yirong
Cai, Xinxia
author_sort Xu, Zhaojie
collection PubMed
description Epilepsy severely impairs the cognitive behavior of patients. It remains unclear whether epilepsy-induced cognitive impairment is associated with neuronal activities in the medial entorhinal cortex (MEC), a region known for its involvement in spatial cognition. To explore this neural mechanism, we recorded the spikes and local field potentials from MEC neurons in lithium–pilocarpine-induced epileptic rats using self-designed microelectrode arrays. Through the open field test, we identified spatial cells exhibiting spatially selective firing properties and assessed their spatial representations in relation to the progression of epilepsy. Meanwhile, we analyzed theta oscillations and theta modulation in both excitatory and inhibitory neurons. Furthermore, we used a novel object recognition test to evaluate changes in spatial cognitive ability of epileptic rats. After the epilepsy modeling, the spatial tuning of various types of spatial cells had suffered a rapid and pronounced damage during the latent period (1 to 5 d). Subsequently, the firing characteristics and theta oscillations were impaired. In the chronic period (>10 d), the performance in the novel object experiment deteriorated. In conclusion, our study demonstrates the detrimental effect on spatial representations and electrophysiological properties of MEC neurons in the epileptic latency, suggesting the potential use of these changes as a “functional biomarker” for predicting cognitive impairment caused by epilepsy.
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spelling pubmed-105039932023-09-16 Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays Xu, Zhaojie Mo, Fan Yang, Gucheng Fan, Penghui Lu, Botao Liang, Wei Kong, Fanli Jing, Luyi Xu, Wei Liu, Juntao Wang, Mixia Wu, Yirong Cai, Xinxia Research (Wash D C) Research Article Epilepsy severely impairs the cognitive behavior of patients. It remains unclear whether epilepsy-induced cognitive impairment is associated with neuronal activities in the medial entorhinal cortex (MEC), a region known for its involvement in spatial cognition. To explore this neural mechanism, we recorded the spikes and local field potentials from MEC neurons in lithium–pilocarpine-induced epileptic rats using self-designed microelectrode arrays. Through the open field test, we identified spatial cells exhibiting spatially selective firing properties and assessed their spatial representations in relation to the progression of epilepsy. Meanwhile, we analyzed theta oscillations and theta modulation in both excitatory and inhibitory neurons. Furthermore, we used a novel object recognition test to evaluate changes in spatial cognitive ability of epileptic rats. After the epilepsy modeling, the spatial tuning of various types of spatial cells had suffered a rapid and pronounced damage during the latent period (1 to 5 d). Subsequently, the firing characteristics and theta oscillations were impaired. In the chronic period (>10 d), the performance in the novel object experiment deteriorated. In conclusion, our study demonstrates the detrimental effect on spatial representations and electrophysiological properties of MEC neurons in the epileptic latency, suggesting the potential use of these changes as a “functional biomarker” for predicting cognitive impairment caused by epilepsy. AAAS 2023-09-15 /pmc/articles/PMC10503993/ /pubmed/37719050 http://dx.doi.org/10.34133/research.0229 Text en Copyright © 2023 Zhaojie Xu et al. https://creativecommons.org/licenses/by/4.0/Exclusive licensee Science and Technology Review Publishing House. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Xu, Zhaojie
Mo, Fan
Yang, Gucheng
Fan, Penghui
Lu, Botao
Liang, Wei
Kong, Fanli
Jing, Luyi
Xu, Wei
Liu, Juntao
Wang, Mixia
Wu, Yirong
Cai, Xinxia
Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays
title Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays
title_full Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays
title_fullStr Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays
title_full_unstemmed Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays
title_short Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays
title_sort impaired spatial firing representations of neurons in the medial entorhinal cortex of the epileptic rat using microelectrode arrays
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10503993/
https://www.ncbi.nlm.nih.gov/pubmed/37719050
http://dx.doi.org/10.34133/research.0229
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