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Evaluation of photochemically cross-linked collagen/gold nanoparticle composites as potential skin tissue scaffolds

Collagen type I is the main structural unit in skin tissue and is therefore used preferentially in skin tissue scaffolds. However, collagen-based 3D scaffolds have weak aqueous stability and degradation profiles in their uncross-linked states and chemical cross-linking reagents arise toxicity concer...

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Autores principales: YELKUVAN, Evrim Meriç, ERDEMLİ, Özge, YILMAZ, Bengi, AKTÜRK, Ömer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Scientific and Technological Research Council of Turkey (TUBITAK) 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10504012/
https://www.ncbi.nlm.nih.gov/pubmed/37720862
http://dx.doi.org/10.55730/1300-0527.3521
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author YELKUVAN, Evrim Meriç
ERDEMLİ, Özge
YILMAZ, Bengi
AKTÜRK, Ömer
author_facet YELKUVAN, Evrim Meriç
ERDEMLİ, Özge
YILMAZ, Bengi
AKTÜRK, Ömer
author_sort YELKUVAN, Evrim Meriç
collection PubMed
description Collagen type I is the main structural unit in skin tissue and is therefore used preferentially in skin tissue scaffolds. However, collagen-based 3D scaffolds have weak aqueous stability and degradation profiles in their uncross-linked states and chemical cross-linking reagents arise toxicity concerns, which generally restrict the spectrum of their biomedical applicability. Here, the research goal is to photochemically cross-link collagen type I with rose bengal (RB) when subjected to green laser light and to investigate the effect of silk sericin-capped gold nanoparticles (S-AuNP) when incorporated into scaffolds on the cross-linking process and thus on the scaffold properties. All the collagen scaffolds, that is plain collagen (C), collagen/S-AuNP (C-Au), cross-linked collagen (C-RBL), and cross-linked collagen/S-AuNP (C-AuRBL) were characterized for their potential as skin tissue scaffolds. C-AuRBL group had the best thermal stability, resistance to enzymatic degradation, and more uniform pore size distribution. None of the groups had cytotoxicity (cell viability > 70%) regarding the microscopic observations and MTT cell viability assays for L929 fibroblasts. L929 fibroblasts and primary adult human epidermal keratinocytes (HEKa) were also separately seeded on C-AuRBL scaffolds and according to microscopy results, they could support the stimulation of adhesion, morphological changes, and spreading of both cells, thereby encouraging the usage of this fabrication strategy for prospective skin tissue scaffolds.
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spelling pubmed-105040122023-09-16 Evaluation of photochemically cross-linked collagen/gold nanoparticle composites as potential skin tissue scaffolds YELKUVAN, Evrim Meriç ERDEMLİ, Özge YILMAZ, Bengi AKTÜRK, Ömer Turk J Chem Research Article Collagen type I is the main structural unit in skin tissue and is therefore used preferentially in skin tissue scaffolds. However, collagen-based 3D scaffolds have weak aqueous stability and degradation profiles in their uncross-linked states and chemical cross-linking reagents arise toxicity concerns, which generally restrict the spectrum of their biomedical applicability. Here, the research goal is to photochemically cross-link collagen type I with rose bengal (RB) when subjected to green laser light and to investigate the effect of silk sericin-capped gold nanoparticles (S-AuNP) when incorporated into scaffolds on the cross-linking process and thus on the scaffold properties. All the collagen scaffolds, that is plain collagen (C), collagen/S-AuNP (C-Au), cross-linked collagen (C-RBL), and cross-linked collagen/S-AuNP (C-AuRBL) were characterized for their potential as skin tissue scaffolds. C-AuRBL group had the best thermal stability, resistance to enzymatic degradation, and more uniform pore size distribution. None of the groups had cytotoxicity (cell viability > 70%) regarding the microscopic observations and MTT cell viability assays for L929 fibroblasts. L929 fibroblasts and primary adult human epidermal keratinocytes (HEKa) were also separately seeded on C-AuRBL scaffolds and according to microscopy results, they could support the stimulation of adhesion, morphological changes, and spreading of both cells, thereby encouraging the usage of this fabrication strategy for prospective skin tissue scaffolds. Scientific and Technological Research Council of Turkey (TUBITAK) 2022-10-08 /pmc/articles/PMC10504012/ /pubmed/37720862 http://dx.doi.org/10.55730/1300-0527.3521 Text en © TÜBİTAK https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
YELKUVAN, Evrim Meriç
ERDEMLİ, Özge
YILMAZ, Bengi
AKTÜRK, Ömer
Evaluation of photochemically cross-linked collagen/gold nanoparticle composites as potential skin tissue scaffolds
title Evaluation of photochemically cross-linked collagen/gold nanoparticle composites as potential skin tissue scaffolds
title_full Evaluation of photochemically cross-linked collagen/gold nanoparticle composites as potential skin tissue scaffolds
title_fullStr Evaluation of photochemically cross-linked collagen/gold nanoparticle composites as potential skin tissue scaffolds
title_full_unstemmed Evaluation of photochemically cross-linked collagen/gold nanoparticle composites as potential skin tissue scaffolds
title_short Evaluation of photochemically cross-linked collagen/gold nanoparticle composites as potential skin tissue scaffolds
title_sort evaluation of photochemically cross-linked collagen/gold nanoparticle composites as potential skin tissue scaffolds
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10504012/
https://www.ncbi.nlm.nih.gov/pubmed/37720862
http://dx.doi.org/10.55730/1300-0527.3521
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