Cargando…

Systematic review of mRNA expression in human oocytes: understanding the molecular mechanisms underlying oocyte competence

The biggest cell in the human body, the oocyte, encloses almost the complete machinery to start life. Despite all the research performed to date, defining oocyte quality is still a major goal of reproductive science. It is the consensus that mature oocytes are transcriptionally silent although, duri...

Descripción completa

Detalles Bibliográficos
Autores principales: Gonzalez, Xavier Viñals, Almutlaq, Arwa, Gupta, Sioban Sen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10504133/
https://www.ncbi.nlm.nih.gov/pubmed/37558907
http://dx.doi.org/10.1007/s10815-023-02906-9
_version_ 1785106661294211072
author Gonzalez, Xavier Viñals
Almutlaq, Arwa
Gupta, Sioban Sen
author_facet Gonzalez, Xavier Viñals
Almutlaq, Arwa
Gupta, Sioban Sen
author_sort Gonzalez, Xavier Viñals
collection PubMed
description The biggest cell in the human body, the oocyte, encloses almost the complete machinery to start life. Despite all the research performed to date, defining oocyte quality is still a major goal of reproductive science. It is the consensus that mature oocytes are transcriptionally silent although, during their growth, the cell goes through stages of active transcription and translation, which will endow the oocyte with the competence to undergo nuclear maturation, and the oocyte and embryo to initiate timely translation before the embryonic genome is fully activated (cytoplasmic maturation). A systematic search was conducted across three electronic databases and the literature was critically appraised using the KMET score system. The aim was to identify quantitative differences in transcriptome of human oocytes that may link to patient demographics that could affect oocyte competence. Data was analysed following the principles of thematic analysis. Differences in the transcriptome were identified with respect to age or pathological conditions and affected chromosome mis segregation, perturbations of the nuclear envelope, premature maturation, and alterations in metabolic pathways—amongst others—in human oocytes.
format Online
Article
Text
id pubmed-10504133
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-105041332023-09-17 Systematic review of mRNA expression in human oocytes: understanding the molecular mechanisms underlying oocyte competence Gonzalez, Xavier Viñals Almutlaq, Arwa Gupta, Sioban Sen J Assist Reprod Genet Review The biggest cell in the human body, the oocyte, encloses almost the complete machinery to start life. Despite all the research performed to date, defining oocyte quality is still a major goal of reproductive science. It is the consensus that mature oocytes are transcriptionally silent although, during their growth, the cell goes through stages of active transcription and translation, which will endow the oocyte with the competence to undergo nuclear maturation, and the oocyte and embryo to initiate timely translation before the embryonic genome is fully activated (cytoplasmic maturation). A systematic search was conducted across three electronic databases and the literature was critically appraised using the KMET score system. The aim was to identify quantitative differences in transcriptome of human oocytes that may link to patient demographics that could affect oocyte competence. Data was analysed following the principles of thematic analysis. Differences in the transcriptome were identified with respect to age or pathological conditions and affected chromosome mis segregation, perturbations of the nuclear envelope, premature maturation, and alterations in metabolic pathways—amongst others—in human oocytes. Springer US 2023-08-10 2023-10 /pmc/articles/PMC10504133/ /pubmed/37558907 http://dx.doi.org/10.1007/s10815-023-02906-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review
Gonzalez, Xavier Viñals
Almutlaq, Arwa
Gupta, Sioban Sen
Systematic review of mRNA expression in human oocytes: understanding the molecular mechanisms underlying oocyte competence
title Systematic review of mRNA expression in human oocytes: understanding the molecular mechanisms underlying oocyte competence
title_full Systematic review of mRNA expression in human oocytes: understanding the molecular mechanisms underlying oocyte competence
title_fullStr Systematic review of mRNA expression in human oocytes: understanding the molecular mechanisms underlying oocyte competence
title_full_unstemmed Systematic review of mRNA expression in human oocytes: understanding the molecular mechanisms underlying oocyte competence
title_short Systematic review of mRNA expression in human oocytes: understanding the molecular mechanisms underlying oocyte competence
title_sort systematic review of mrna expression in human oocytes: understanding the molecular mechanisms underlying oocyte competence
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10504133/
https://www.ncbi.nlm.nih.gov/pubmed/37558907
http://dx.doi.org/10.1007/s10815-023-02906-9
work_keys_str_mv AT gonzalezxaviervinals systematicreviewofmrnaexpressioninhumanoocytesunderstandingthemolecularmechanismsunderlyingoocytecompetence
AT almutlaqarwa systematicreviewofmrnaexpressioninhumanoocytesunderstandingthemolecularmechanismsunderlyingoocytecompetence
AT guptasiobansen systematicreviewofmrnaexpressioninhumanoocytesunderstandingthemolecularmechanismsunderlyingoocytecompetence