Optimization of Cas12a for multiplexed genome-scale transcriptional activation

Cas12a CRISPR technology, unlike Cas9, allows for facile multiplexing of guide RNAs from a single transcript, simplifying combinatorial perturbations. While Cas12a has been implemented for multiplexed knockout genetic screens, it has yet to be optimized for CRISPR activation (CRISPRa) screens in hum...

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Detalles Bibliográficos
Autores principales: Griffith, Audrey L., Zheng, Fengyi, McGee, Abby V., Miller, Nathan W., Szegletes, Zsofia M., Reint, Ganna, Gademann, Fabian, Nwolah, Ifunanya, Hegde, Mudra, Liu, Yanjing V., Goodale, Amy, Doench, John G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Technology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10504673/
https://www.ncbi.nlm.nih.gov/pubmed/37719144
http://dx.doi.org/10.1016/j.xgen.2023.100387

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10504673/
https://www.ncbi.nlm.nih.gov/pubmed/37719144
http://dx.doi.org/10.1016/j.xgen.2023.100387

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