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Optimization of Cas12a for multiplexed genome-scale transcriptional activation

Cas12a CRISPR technology, unlike Cas9, allows for facile multiplexing of guide RNAs from a single transcript, simplifying combinatorial perturbations. While Cas12a has been implemented for multiplexed knockout genetic screens, it has yet to be optimized for CRISPR activation (CRISPRa) screens in hum...

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Detalles Bibliográficos
Autores principales:	Griffith, Audrey L., Zheng, Fengyi, McGee, Abby V., Miller, Nathan W., Szegletes, Zsofia M., Reint, Ganna, Gademann, Fabian, Nwolah, Ifunanya, Hegde, Mudra, Liu, Yanjing V., Goodale, Amy, Doench, John G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Technology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10504673/ https://www.ncbi.nlm.nih.gov/pubmed/37719144 http://dx.doi.org/10.1016/j.xgen.2023.100387

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