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The time course of nod-like receptor family pyrin domain containing 3 inflammasome complex expressions in the testis tissue of an experimental varicocele rat model: An experimental study
BACKGROUND: Varicocele is characterized by abnormal dilation of the testicular vein, which results in hypoxia, the accumulation of reactive oxygen species, and the production of proinflammatory cytokines. It seems that a group of cytosolic receptors named nod-like receptor family, pyrin domain conta...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Knowledge E
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505694/ https://www.ncbi.nlm.nih.gov/pubmed/37727396 http://dx.doi.org/10.18502/ijrm.v21i7.13895 |
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author | Fallah Asl, Hamideh Jalali Mashayekhi, Farideh Zendedel, Adib Baazm, Maryam |
author_facet | Fallah Asl, Hamideh Jalali Mashayekhi, Farideh Zendedel, Adib Baazm, Maryam |
author_sort | Fallah Asl, Hamideh |
collection | PubMed |
description | BACKGROUND: Varicocele is characterized by abnormal dilation of the testicular vein, which results in hypoxia, the accumulation of reactive oxygen species, and the production of proinflammatory cytokines. It seems that a group of cytosolic receptors named nod-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome, is activated and involved in the pathogenesis of varicocele. OBJECTIVE: We aim to determine the time course of NLRP3 inflammasome expression in the testis tissue following varicocele induction. MATERIALS AND METHODS: In this experimental study, 36 adult Wistar rats (8 wk, 200-250 gr) were used. For the varicocele induction, the left renal vein was partially ligated. The mRNA levels of NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain, and caspase-1 were evaluated by real-time polymerase chain reaction at 1, 2, 4, 8, and 12 wk after varicocele induction. RESULTS: Results showed that the gene expression of NLRP3 inflammasome component including NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain, and caspase-1 did not alter during week 1, 2, 4, and 8 after operation (p = 0.09). 12 wk after varicocele induction, gene expression levels were significantly up-regulated (p = 0.02). CONCLUSION: Our data provides clear evidence that varicocele stimulates inflammasome activation in the testis tissue 12 wk after the operation, and this time is required for investigating NLRP3 activity in the varicocele rat model. |
format | Online Article Text |
id | pubmed-10505694 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Knowledge E |
record_format | MEDLINE/PubMed |
spelling | pubmed-105056942023-09-19 The time course of nod-like receptor family pyrin domain containing 3 inflammasome complex expressions in the testis tissue of an experimental varicocele rat model: An experimental study Fallah Asl, Hamideh Jalali Mashayekhi, Farideh Zendedel, Adib Baazm, Maryam Int J Reprod Biomed Original Article BACKGROUND: Varicocele is characterized by abnormal dilation of the testicular vein, which results in hypoxia, the accumulation of reactive oxygen species, and the production of proinflammatory cytokines. It seems that a group of cytosolic receptors named nod-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome, is activated and involved in the pathogenesis of varicocele. OBJECTIVE: We aim to determine the time course of NLRP3 inflammasome expression in the testis tissue following varicocele induction. MATERIALS AND METHODS: In this experimental study, 36 adult Wistar rats (8 wk, 200-250 gr) were used. For the varicocele induction, the left renal vein was partially ligated. The mRNA levels of NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain, and caspase-1 were evaluated by real-time polymerase chain reaction at 1, 2, 4, 8, and 12 wk after varicocele induction. RESULTS: Results showed that the gene expression of NLRP3 inflammasome component including NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain, and caspase-1 did not alter during week 1, 2, 4, and 8 after operation (p = 0.09). 12 wk after varicocele induction, gene expression levels were significantly up-regulated (p = 0.02). CONCLUSION: Our data provides clear evidence that varicocele stimulates inflammasome activation in the testis tissue 12 wk after the operation, and this time is required for investigating NLRP3 activity in the varicocele rat model. Knowledge E 2023-08-23 /pmc/articles/PMC10505694/ /pubmed/37727396 http://dx.doi.org/10.18502/ijrm.v21i7.13895 Text en Copyright © 2023 Fallah Asl et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Fallah Asl, Hamideh Jalali Mashayekhi, Farideh Zendedel, Adib Baazm, Maryam The time course of nod-like receptor family pyrin domain containing 3 inflammasome complex expressions in the testis tissue of an experimental varicocele rat model: An experimental study |
title | The time course of nod-like receptor family pyrin domain containing 3 inflammasome complex expressions in the testis tissue of an experimental varicocele rat model: An experimental study |
title_full | The time course of nod-like receptor family pyrin domain containing 3 inflammasome complex expressions in the testis tissue of an experimental varicocele rat model: An experimental study |
title_fullStr | The time course of nod-like receptor family pyrin domain containing 3 inflammasome complex expressions in the testis tissue of an experimental varicocele rat model: An experimental study |
title_full_unstemmed | The time course of nod-like receptor family pyrin domain containing 3 inflammasome complex expressions in the testis tissue of an experimental varicocele rat model: An experimental study |
title_short | The time course of nod-like receptor family pyrin domain containing 3 inflammasome complex expressions in the testis tissue of an experimental varicocele rat model: An experimental study |
title_sort | time course of nod-like receptor family pyrin domain containing 3 inflammasome complex expressions in the testis tissue of an experimental varicocele rat model: an experimental study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505694/ https://www.ncbi.nlm.nih.gov/pubmed/37727396 http://dx.doi.org/10.18502/ijrm.v21i7.13895 |
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