An in vitro Assay to Probe the Formation of Biomolecular Condensates

Biomolecular condensates are membrane-less assemblies of proteins and nucleic acids formed through liquid–liquid phase separation (LLPS). These assemblies are known to temporally and spatially regulate numerous biological activities and cellular processes in plants and animals. In vitro phase separa...

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Detalles Bibliográficos
Autores principales: Zhang, Yu, Shen, Lisha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505955/
https://www.ncbi.nlm.nih.gov/pubmed/37727870
http://dx.doi.org/10.21769/BioProtoc.4813
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author Zhang, Yu
Shen, Lisha
author_facet Zhang, Yu
Shen, Lisha
author_sort Zhang, Yu
collection PubMed
description Biomolecular condensates are membrane-less assemblies of proteins and nucleic acids formed through liquid–liquid phase separation (LLPS). These assemblies are known to temporally and spatially regulate numerous biological activities and cellular processes in plants and animals. In vitro phase separation assay using recombinant proteins represents one of the standard ways to examine the properties of proteins undergoing LLPS. Here, we present a detailed protocol to investigate in vitro LLPS using in vitro expressed and purified recombinant proteins.
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spelling pubmed-105059552023-09-19 An in vitro Assay to Probe the Formation of Biomolecular Condensates Zhang, Yu Shen, Lisha Bio Protoc Methods Article Biomolecular condensates are membrane-less assemblies of proteins and nucleic acids formed through liquid–liquid phase separation (LLPS). These assemblies are known to temporally and spatially regulate numerous biological activities and cellular processes in plants and animals. In vitro phase separation assay using recombinant proteins represents one of the standard ways to examine the properties of proteins undergoing LLPS. Here, we present a detailed protocol to investigate in vitro LLPS using in vitro expressed and purified recombinant proteins. Bio-Protocol 2023-09-05 /pmc/articles/PMC10505955/ /pubmed/37727870 http://dx.doi.org/10.21769/BioProtoc.4813 Text en ©Copyright : © 2023 The Authors; This is an open access article under the CC BY-NC license https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
spellingShingle Methods Article
Zhang, Yu
Shen, Lisha
An in vitro Assay to Probe the Formation of Biomolecular Condensates
title An in vitro Assay to Probe the Formation of Biomolecular Condensates
title_full An in vitro Assay to Probe the Formation of Biomolecular Condensates
title_fullStr An in vitro Assay to Probe the Formation of Biomolecular Condensates
title_full_unstemmed An in vitro Assay to Probe the Formation of Biomolecular Condensates
title_short An in vitro Assay to Probe the Formation of Biomolecular Condensates
title_sort in vitro assay to probe the formation of biomolecular condensates
topic Methods Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505955/
https://www.ncbi.nlm.nih.gov/pubmed/37727870
http://dx.doi.org/10.21769/BioProtoc.4813
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