Cargando…

Tibial cortex transverse transport potentiates diabetic wound healing via activation of SDF-1/CXCR4 signaling

BACKGROUND: The current treatments for diabetic foot ulcers have disadvantages of slow action and numerous complications. Tibial cortex transverse transport (TTT) surgery is an extension of the Ilizarov technique used to treat diabetic foot ulcers, and can shorten the repair time of diabetic foot ul...

Descripción completa

Detalles Bibliográficos
Autores principales: Ou, Shuanji, Wu, Xiaodong, Yang, Yang, Xia, Changliang, Zhang, Wei, Qu, Yudun, Li, Jiaxuan, Chen, Bo, Zhu, Lilin, Xu, Changpeng, Qi, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10506586/
https://www.ncbi.nlm.nih.gov/pubmed/37727693
http://dx.doi.org/10.7717/peerj.15894
_version_ 1785107143364444160
author Ou, Shuanji
Wu, Xiaodong
Yang, Yang
Xia, Changliang
Zhang, Wei
Qu, Yudun
Li, Jiaxuan
Chen, Bo
Zhu, Lilin
Xu, Changpeng
Qi, Yong
author_facet Ou, Shuanji
Wu, Xiaodong
Yang, Yang
Xia, Changliang
Zhang, Wei
Qu, Yudun
Li, Jiaxuan
Chen, Bo
Zhu, Lilin
Xu, Changpeng
Qi, Yong
author_sort Ou, Shuanji
collection PubMed
description BACKGROUND: The current treatments for diabetic foot ulcers have disadvantages of slow action and numerous complications. Tibial cortex transverse transport (TTT) surgery is an extension of the Ilizarov technique used to treat diabetic foot ulcers, and can shorten the repair time of diabetic foot ulcers. This study assessed the TTT technique for its effectiveness in healing diabetic foot ulcer skin lesions and its related molecular mechanisms. METHODS: Diabetic rat models were established by injecting healthy Sprague-Dawley rats with streptozotocin (STZ). The effects of TTT surgery on the model rats were assessed by recording changes in body weight, analyzing skin wound pictures, and performing H&E staining to assess the recovery of wounded skin. The numbers of endothelial progenitor cells (EPCs) in peripheral blood were analyzed by flow cytometry, and levels of CXCR4 and SDF-1 expression were qualitatively analyzed by immunofluorescence, immunohistochemistry, qRT-PCR, and western blotting. RESULTS: Both the histological results and foot wound pictures indicated that TTT promoted diabetic wound healing. Flow cytometry results showed that TTT increased the numbers of EPCs in peripheral blood as determined by CD34 and CD133 expression. In addition, activation of the SDF-1/CXCR4 signaling pathway and an accumulation of EPCs were observed in skin ulcers sites after TTT surgery. Finally, the levels of SDF-1 and CXCR4 mRNA and protein expression in the TTT group were higher than those in a blank or fixator group. CONCLUSION: TTT promoted skin wound healing in diabetic foot ulcers possibly by activating the SDF-1/CXCR4 signaling pathway.
format Online
Article
Text
id pubmed-10506586
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-105065862023-09-19 Tibial cortex transverse transport potentiates diabetic wound healing via activation of SDF-1/CXCR4 signaling Ou, Shuanji Wu, Xiaodong Yang, Yang Xia, Changliang Zhang, Wei Qu, Yudun Li, Jiaxuan Chen, Bo Zhu, Lilin Xu, Changpeng Qi, Yong PeerJ Biochemistry BACKGROUND: The current treatments for diabetic foot ulcers have disadvantages of slow action and numerous complications. Tibial cortex transverse transport (TTT) surgery is an extension of the Ilizarov technique used to treat diabetic foot ulcers, and can shorten the repair time of diabetic foot ulcers. This study assessed the TTT technique for its effectiveness in healing diabetic foot ulcer skin lesions and its related molecular mechanisms. METHODS: Diabetic rat models were established by injecting healthy Sprague-Dawley rats with streptozotocin (STZ). The effects of TTT surgery on the model rats were assessed by recording changes in body weight, analyzing skin wound pictures, and performing H&E staining to assess the recovery of wounded skin. The numbers of endothelial progenitor cells (EPCs) in peripheral blood were analyzed by flow cytometry, and levels of CXCR4 and SDF-1 expression were qualitatively analyzed by immunofluorescence, immunohistochemistry, qRT-PCR, and western blotting. RESULTS: Both the histological results and foot wound pictures indicated that TTT promoted diabetic wound healing. Flow cytometry results showed that TTT increased the numbers of EPCs in peripheral blood as determined by CD34 and CD133 expression. In addition, activation of the SDF-1/CXCR4 signaling pathway and an accumulation of EPCs were observed in skin ulcers sites after TTT surgery. Finally, the levels of SDF-1 and CXCR4 mRNA and protein expression in the TTT group were higher than those in a blank or fixator group. CONCLUSION: TTT promoted skin wound healing in diabetic foot ulcers possibly by activating the SDF-1/CXCR4 signaling pathway. PeerJ Inc. 2023-09-15 /pmc/articles/PMC10506586/ /pubmed/37727693 http://dx.doi.org/10.7717/peerj.15894 Text en ©2023 Ou et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biochemistry
Ou, Shuanji
Wu, Xiaodong
Yang, Yang
Xia, Changliang
Zhang, Wei
Qu, Yudun
Li, Jiaxuan
Chen, Bo
Zhu, Lilin
Xu, Changpeng
Qi, Yong
Tibial cortex transverse transport potentiates diabetic wound healing via activation of SDF-1/CXCR4 signaling
title Tibial cortex transverse transport potentiates diabetic wound healing via activation of SDF-1/CXCR4 signaling
title_full Tibial cortex transverse transport potentiates diabetic wound healing via activation of SDF-1/CXCR4 signaling
title_fullStr Tibial cortex transverse transport potentiates diabetic wound healing via activation of SDF-1/CXCR4 signaling
title_full_unstemmed Tibial cortex transverse transport potentiates diabetic wound healing via activation of SDF-1/CXCR4 signaling
title_short Tibial cortex transverse transport potentiates diabetic wound healing via activation of SDF-1/CXCR4 signaling
title_sort tibial cortex transverse transport potentiates diabetic wound healing via activation of sdf-1/cxcr4 signaling
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10506586/
https://www.ncbi.nlm.nih.gov/pubmed/37727693
http://dx.doi.org/10.7717/peerj.15894
work_keys_str_mv AT oushuanji tibialcortextransversetransportpotentiatesdiabeticwoundhealingviaactivationofsdf1cxcr4signaling
AT wuxiaodong tibialcortextransversetransportpotentiatesdiabeticwoundhealingviaactivationofsdf1cxcr4signaling
AT yangyang tibialcortextransversetransportpotentiatesdiabeticwoundhealingviaactivationofsdf1cxcr4signaling
AT xiachangliang tibialcortextransversetransportpotentiatesdiabeticwoundhealingviaactivationofsdf1cxcr4signaling
AT zhangwei tibialcortextransversetransportpotentiatesdiabeticwoundhealingviaactivationofsdf1cxcr4signaling
AT quyudun tibialcortextransversetransportpotentiatesdiabeticwoundhealingviaactivationofsdf1cxcr4signaling
AT lijiaxuan tibialcortextransversetransportpotentiatesdiabeticwoundhealingviaactivationofsdf1cxcr4signaling
AT chenbo tibialcortextransversetransportpotentiatesdiabeticwoundhealingviaactivationofsdf1cxcr4signaling
AT zhulilin tibialcortextransversetransportpotentiatesdiabeticwoundhealingviaactivationofsdf1cxcr4signaling
AT xuchangpeng tibialcortextransversetransportpotentiatesdiabeticwoundhealingviaactivationofsdf1cxcr4signaling
AT qiyong tibialcortextransversetransportpotentiatesdiabeticwoundhealingviaactivationofsdf1cxcr4signaling